Kinematic calibration plays a pivotal role in enhancing the absolute positioning accuracy of industrial robots, with parameter identification and error compensation constituting its core components. While the conventional parameter identification method, based on linearization, has shown promise, it suffers from the loss of high-order system information. To address this issue, we propose an unscented Kalman filter (UKF) with adaptive process noise covariance for robot kinematic parameter identification. The kinematic model of a typical 6-degree-of-freedom industrial robot is established. The UKF is introduced to identify the unknown constant parameters within this model. To mitigate the reliance of the UKF on the process noise covariance, an adaptive process noise covariance strategy is proposed to adjust and correct this covariance. The effectiveness of the proposed algorithm is then demonstrated through identification and error compensation experiments for the industrial robot. Results indicate its superior stability and accuracy across various initial conditions. Compared to the conventional UKF algorithm, the proposed approach enhances the robot’s accuracy stability by 25% under differing initial conditions. Moreover, compared to alternative methods such as the extended Kalman algorithm, particle swarm optimization algorithm, and grey wolf algorithm, the proposed approach yields average improvements of 4.13%, 26.47%, and 41.59%, respectively. Full article

Blood–brain barrier (BBB) dysfunction is a key feature in neuroimmunological and neurodegenerative diseases. In this study, we developed a microfluidic human BBB-on-a-chip to model barrier dysfunction and immune cell migration using immortalized TY10 brain endothelial cells, pericytes, and astrocytes. It was found that immortalized TY10 brain endothelial cells developed a microvascular structure under flow. Pericytes were localized on the basal side surrounding the TY10 microvascular structure, showing an in vivo-like structure. Barrier integrity increased under co-culture with pericytes. In addition, both ethylenediaminetetraacetic acid (EDTA) and anti-Claudin-5 (CLDN5) neutralizing antibody caused a decrease in the transendothelial electrical resistance (TEER). EDTA caused the leakage of 20 kDa dextran, suggesting different effects on the BBB based on the mechanism of action, whereas anti-CLDN5 antibody did not cause leakage. In the tri-culture model, human T cells migrated through endothelial vessels towards basal C-X-C motif chemokine ligand 12 (CXCL12). The live-imaging analysis confirmed the extravasation of fluorescence-labelled T cells in a CXCL12-concentration- and time-dependent manner. Our BBB model had an in vivo-like structure and successfully represented barrier dysfunction and transendothelial T cell migration. In addition, our study suggests that the inhibition of CLDN5 attenuates the BBB in humans. This platform has various potential uses in relation to the BBB in both drug discovery research and in elucidating the mechanisms of central nervous system diseases. Full article

Fusarium wilt of banana (FWB) is the most limiting disease in this crop. The phytosanitary emergency caused by FWB since 2019 in Colombia has required the development of ecofriendly control methods. The aim of this study was to test the effectiveness of microbial-based biofungicides against FWB caused by Fusarium oxysporum f. sp. cubense race 1 (Foc R1) and correlate such effect with plant physiological parameters. Five Trichoderma (T1 to T4 and T9) and four Bacillus (T5 to T8)-based biofungicides were evaluated in pot experiments. In vitro, dual confrontation tests were also carried out to test whether the in vitro effects on Foc growth were consistent with the in vivo effects. While Trichoderma-based T3, T4, and T9, and Bacillus-based T8, significantly reduced the growth of Foc R1 in vitro, Trichoderma-based T1, T3, T4, and T9 temporarily reduced the Foc population in the soil. However, the incidence progress of FWB was significantly reduced by Bacterial-based T7 (74% efficacy) and Trichoderma-based T2 (50% efficacy). The molecular analysis showed that T7 prevented the inner tissue colonization by Foc R1 in 80% of inoculated plants. The T2, T4, T7, and T9 treatments mitigated the negative effects caused by Foc R1 on plant physiology and growth. Our data allowed us to identify three promising treatments to control FWB, reducing the progress of the disease, delaying the colonization of inner tissue, and mitigating physiological damages. Further studies should be addressed to determine the modes of action of the biocontrol agents against Foc and validate the utilization in the field. Full article

The main goal of this study was to assess the bioactive and polysaccharide compositions, along with the antioxidant and antibacterial potentials, of five seaweeds collected from the northeastern coast of Algeria. Through Fourier transform infrared spectroscopy analysis and X-ray fluorescence spectroscopy, the study investigated the elemental composition of these seaweeds and their chemical structure. In addition, this study compared and identified the biochemical makeup of the collected seaweed by using cutting-edge methods like tandem mass spectrometry and ultra-high-performance liquid chromatography, and it searched for new sources of nutritionally valuable compounds. According to the study’s findings, Sargassum muticum contains the highest levels of extractable bioactive compounds, showing a phenolic compound content of 235.67 ± 1.13 µg GAE·mg⁻¹ and a total sugar content of 46.43 ± 0.12% DW. Both S. muticum and Dictyota dichotoma have high concentrations of good polyphenols, such as vanillin and chrysin. Another characteristic that sets brown algae apart is their composition. It showed that Cladophora laetevirens has an extracted bioactive compound content of 12.07% and a high capacity to scavenge ABTS⁺ radicals with a value of 78.65 ± 0.96 µg·mL⁻¹, indicating high antioxidant activity. In terms of antibacterial activity, S. muticum seaweed showed excellent growth inhibition. In conclusion, all five species of seaweed under investigation exhibited unique strengths, highlighting the variety of advantageous characteristics of these seaweeds, especially S. muticum. Full article

The majority of observations and criteria related to brain injuries predominantly focus on acceleration and forces, leaving the understanding of the brain in the frequency domain relatively limited. The impact of an injury can be more profound when considering the brain’s resonant frequencies in conjunction with external applied loading and motion. This paper employs a finite element method to conduct an analysis of a human brain under impacts from various angles on the human head. A numerical technique, specifically dynamic mode decomposition (DMD), is utilized to extract modal properties for brain tissue in regions proximate to the corpus callosum and brain stem. Three distinct modal frequencies have been identified, spanning the ranges of 44–68 Hz, 68–155 Hz, and 114–299 Hz. The findings underscore the significance of impact angle, displacement direction, and the specific region of the brain in influencing the modal response of brain tissue during an impact event. Full article

The present review highlights the potential of insect-based proteins to address the growing need for sustainable and secure food systems. The key findings suggest that edible insects offer a viable and environmentally friendly alternative to traditional livestock, requiring significantly less land, water, and feed while emitting lower levels of greenhouse gases. Insect farming can also reduce waste and recycle nutrients, supporting circular economy models. Nutritionally, insects provide high-quality protein, essential amino acids, and beneficial fats, making them valuable to human diets. Despite these benefits, this review emphasizes the need for comprehensive regulatory frameworks to ensure food safety, manage potential allergenicity, and mitigate contamination risks from pathogens and environmental toxins. Additionally, developing innovative processing technologies can enhance the palatability and marketability of insect-based products, promoting consumer acceptance. This review concludes that with appropriate regulatory support and technological advancements, insect-based proteins have the potential to significantly contribute to global food security and sustainability efforts. Full article

In this study, our research aim was to assess the influence of pumpkin seed flour addition on the antioxidant properties, consumer acceptability, functional properties, and texture of wafers. The in vitro gastrointestinal digestion process was used to assess the effectiveness of fortification in terms of the potential bioavailability of phenolic compounds and peptides. The antioxidant activity of the obtained hydrolysates and potentially bioavailable fractions (≤3.5 kDa) was tested. The highest antiradical activity and Fe²⁺ chelation ability (IC₅₀) were noted for the fraction obtained from wafers with the greatest addition of pumpkin seed flour—Pf4 (0.49 mg/mL for ABTS⁺*, 3.84 mg/mL for DPPH*, and 2.04 mg/mL for Fe²⁺ chelation). The addition of pumpkin seed flour caused the color of the wafers to change to a darker one (24.46% differences in L* between C and P4), which influenced consumer ratings. This study shows that adding pumpkin seed flour increases the peptide and phenolic contents of wafers (1.13 mg/mL and 1.01 mg/mL of peptides and 0.429 mg/mL and 0.351 mg/mL of phenolics for P4 and C hydrolysates, respectively) and enhances their antioxidant activity, with only minimal effects on taste, aroma, crispness, water and fat adsorption capacity, and foaming ability. Fractions ≤3.5 kDa showed greater antioxidative activity than hydrolysates, and the addition of pumpkin seed flour improved these properties. To sum up, pumpkin seeds are a valuable source of antioxidant compounds (phenolic compounds and peptides) and can be used to enrich various products. Full article

The mining industry generates vast quantities of mine tailings on an annual basis. However, due to their limited economic value, a significant portion of these tailings are deposited close to mining sites, often underwater. The principal environmental apprehensions associated with mine tailings revolve around their elevated levels of heavy metals and sulfidic minerals. The oxidation of these sulfidic minerals can lead to the formation of acid mine drainage, which in turn releases heavy metals into nearby water systems. The effective management of tailing dams requires substantial financial investments for their construction and meticulous control. Consequently, a pressing need exists for stable, sustainable, and economically viable management approaches. One promising method for addressing mine tailings is through alkali activation, a technique that serves as a stabilization process. This approach yields robust, concrete-like structures by utilizing raw materials abundant in aluminum and silicon, which conveniently constitute the primary components of mining residues. This comprehensive review outlines the research on utilizing alkali activation for mine tailings. It delves into the reactivity and chemical attributes of diverse minerals. Numerous mine tailings exhibit an inadequate level of reactivity under alkaline conditions, so various pre-treatment methodologies and their impacts on mineralogy are meticulously explored. Full article

We have designed cell-penetrating peptides that target the leucine zipper transcription factors ATF5, CEBPB and CEBPD and that promote apoptotic death of a wide range of cancer cell types, but not normal cells, in vitro and in vivo. Though such peptides have the potential for clinical application, their mechanisms of action are not fully understood. Here, we show that one such peptide, Dpep, compromises glucose uptake and glycolysis in a cell context-dependent manner (in about two-thirds of cancer lines assessed). These actions are dependent on induction of tumor suppressor TXNIP (thioredoxin-interacting protein) mRNA and protein. Knockdown studies show that TXNIP significantly contributes to apoptotic death in those cancer cells in which it is induced by Dpep. The metabolic actions of Dpep on glycolysis led us to explore combinations of Dpep with clinically approved drugs metformin and atovaquone that inhibit oxidative phosphorylation and that are in trials for cancer treatment. Dpep showed additive to synergistic activities in all lines tested. In summary, we find that Dpep induces TXNIP in a cell context-dependent manner that in turn suppresses glucose uptake and glycolysis and contributes to apoptotic death of a range of cancer cells. Full article

Regenerative endodontics is a developing field involving the restoration of tooth structure and re-vitality of necrotic pulp. One of the most critical clinical considerations for regenerative endodontic procedures is the disinfection of the root canal system, since infection interferes with regeneration, repair, and stem cell activity. In this study, we aimed to provide the synthesis of injectable biopolymeric tissue scaffolds that can be used in routine clinical and regenerative endodontic treatment procedures using Gelatin methacryloyl (GelMA), and to test the antimicrobial efficacy of Gelatin methacryloyl/Silver nanoparticles (GelMA/AgNP), Gelatin methacryloyl/Hyaluronic acid (GelMA/HYA), and Gelatin methacryloyl/hydroxyapatite (GelMA/HA) composite hydrogels against microorganisms that are often encountered in stubborn infections in endodontic microbiology. Injectable biocomposite hydrogels exhibiting effective antimicrobial activity and non-cytotoxic behavior were successfully synthesized. This is also promising for clinical applications of regenerative endodontic procedures with hydrogels, which are proposed based on the collected data. The GelMA hydrogel loaded with hyaluronic acid showed the highest efficacy against Enterococcus faecalis, one of the stubborn bacteria in the root canal. The GelMA hydrogel loaded with hydroxyapatite also showed a significant effect against Candida albicans, which is another bacteria responsible for stubborn infections in the root canal. Full article

The main topics of the presented papers focus on various aspects of maritime operations and security, including anomaly detection in maritime traffic, collision risk assessment, and the use of Automatic Identification System (AIS) data for enhancing maritime safety and surveillance. These papers cover a wide range of subjects within the maritime domain, such as trajectory clustering, kinematic behaviour analysis, Bayesian networks for risk assessment, resilience analysis of shipping networks, and the development of novel methods for detecting abnormal maritime behaviour. The emphasis is on using data-driven approaches, statistical methodologies, and advanced technologies to improve maritime operations and security. Full article

The direct copolymerization of ethylene with polar monomers to produce functional polyolefins continues to be highly appealing due to its simple operation process and controllable product microstructure. Low-cost nickel catalysts have been extensively utilized in academia for the synthesis of polar polyethylenes. However, the development of high-temperature copolymerization catalysts suitable for industrial production conditions remains a significant challenge. Classified by the resultant copolymers, this review provides a comprehensive summary of the research progress in nickel complex catalyzed ethylene-polar monomer copolymerization at elevated temperatures in the past five years. The polymerization results of ethylene–methyl acrylate copolymers, ethylene-tert–butyl acrylate copolymers, ethylene–other fundamental polar monomer copolymers, and ethylene–special polar monomer copolymers are thoroughly summarized. The involved nickel catalysts include the phosphine-phenolate type, bisphosphine-monoxide type, phosphine-carbonyl type, phosphine-benzenamine type, and the phosphine-enolate type. The effective modulation of catalytic activity, molecular weight, molecular weight distribution, melting point, and polar monomer incorporation ratio by these catalysts is concluded and discussed. It reveals that the optimization of the catalyst system is mainly achieved through the methods of catalyst structure rational design, extra additive introduction, and single-site catalyst heterogenization. As a result, some outstanding catalysts are capable of producing polar polyethylenes that closely resemble commercial products. To achieve industrialization, it is essential to further emphasize the fundamental science of high-temperature copolymerization systems and the application performance of resultant polar polyethylenes. Full article

A novel integrated DC-DC converter is proposed for the first stage of two-stage grid connected photovoltaic (PV) systems with energy storage systems. The proposed three-port converter (TPC) consists of a buck–boost converter, interposed between the battery storage system and the DC-AC inverter, in series with PV modules. The buck–boost converter in the proposed TPC is utilized for maximum power point tracking by regulating two power switches. The output power of the proposed converter is regulated by controlling the DC-AC converter. During the battery-charging mode, partial power regulation is employed with a direct power flow path (the series-connection of the PV panel, the battery and the output). As resistances in this path are almost negligible, the power conversion efficiency is higher than existing topologies. During battery-discharging mode, the power conversion is processed through a buck–boost converter with only two active power switches and one inductor. With fewer components, higher power conversion efficiency is also achieved. The circuit operation and analysis are presented in detail. To illustrate the simplicity of the converter control, the performance of the converter is tested with a straightforward maximum power point tracking on a PV system with battery cells. Simulation and experimental tests are carried out to demonstrate circuit operation and power conversion efficiency. Full article

Human error is a mark assigned to an event that has negative effects or does not produce a desired result, with emotions playing an important role in how humans think and behave. If we detect feelings early, it may decrease human error. The human voice is one of the most powerful tools that can be used for emotion recognition. This study aims to reduce human error by building a system that detects positive or negative emotions of a user like (happy, sad, fear, and anger) through the analysis of the proposed vocal emotion component using Convolutional Neural Networks. By applying the proposed method to an emotional voice database (RAVDESS) using Librosa for voice processing and PyTorch, with the emotion classification of (happy/angry), the results show a better accuracy (98%) in comparison to the literature with regard to making a decision to deny or allow a user to access sensitive operations or send a warning to the system administrator prior to accessing system resources. Full article

Microbial mineralization of calcium–magnesium carbonate has been a hot research topic in the fields of geomicrobiology and engineering geology in the past decades. However, the formation and phase transition mechanism of calcium–magnesium carbonate polymorphs at different Mg/Ca ratios still need to be explored. In this study, microbial induced carbonate mineralization experiments were carried out for 50 days in culture medium with Mg/Ca molar ratios of 0, 1.5, and 3 under the action of Curvibacter sp. HJ-1. The roles of bacteria and the Mg/Ca ratio on the mineral formation and phase transition were investigated. Experimental results show that (1) strain HJ-1 could induce vaterite, aragonite, and magnesium calcite formation in culture media with different Mg/Ca molar ratios. The increased stability of the metastable phase suggests that bacterial extracellular secretions and Mg²⁺ ions inhibit the carbonate phase-transition process. (2) The morphology of bacteriological carbonate minerals and the formation mechanism of spherical minerals were different in Mg-free and Mg-containing media. (3) The increased Mg/Ca ratio in the culture medium has an influence on the formation and transformation of calcium–magnesium carbonate by controlling the metabolism of Curvibacter sp. HJ-1 and the activity of bacterial secretion. Full article

Respiratory disorders significantly impact adolescents' health, often resulting in hospital admissions. Meteorological elements such as wind patterns have emerged as potential contributors to respiratory symptoms. However, it remains uncertain whether fluctuations in wind characteristics over extended periods have a tangible impact on respiratory health, particularly in regions characterized by distinct annual wind patterns. Crete is situated in the central-eastern Mediterranean Sea and frequently faces southerly winds carrying Sahara Desert sand from Africa and northerly winds from the Aegean Sea. This retrospective study analyzes long-term wind direction data and their relationship to respiratory symptoms observed in children up to 14 years old admitted at the University Hospital of Heraklion between 2002 and 2010. Symptoms such as headache, dyspnea, dry cough, dizziness, tachypnea, throat ache, and earache were predominantly reported during the presence of southern winds. Fever, productive cough, and chest pain were more frequently reported during northern winds. Cough was the most common symptom regardless of the wind pattern. Southern winds were significantly associated with higher probabilities of productive or non-productive cough, headache, dyspnea, tachypnea, dizziness, earache, and throat ache. Northern winds were related to a higher incidence of productive cough. Rhinitis, asthma, allergies, pharyngitis, and sinusitis were related to southern winds, while bronchiolitis and pneumonia were associated with northern winds. These findings underscore the critical role of local climatic factors, emphasizing their potential impact on exacerbating respiratory conditions in children. Moreover, they point out the need for further research to elucidate the underlying mechanisms and develop targeted interventions for at-risk populations. Full article

This study is the first to investigate the chemical composition and antioxidant, anti-inflammatory, and cytotoxic activities of Peperomia leptostachya leaf oil. A yellow oil was obtained through hydro-distillation, with a yield of 0.1% (w/w). The GC-MS analysis revealed 66 compounds, constituting 99.6% of the oil. Sesquiterpene hydrocarbons predominated (70.4%), followed by monoterpene hydrocarbons (13.2%), oxygenated sesquiterpenes (12.4%), non-terpenic compounds (2.0%), and oxygenated monoterpenes (1.6%). Major constituents included germacrene D (25.1%), (E)-caryophyllene (17.4%), bicyclogermacrene (6.6%), α-pinene (6.2%), and β-pinene (4.7%). The assessment of antioxidant capacity via 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay yielded a weak effect, with an IC₅₀ value > 100 µg/mL. The inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells was quantified using the MTT assay, showing an IC₅₀ value of 15.15 ± 0.68 µg/mL. Furthermore, cytotoxic effects on SK-LU-1 cell line growth were evaluated using the sulforhodamine B assay, resulting in an IC₅₀ value of 37.45 ± 2.43 μg/mL. The anti-inflammatory activity was notable among the analyzed bioactivities of this oil. By employing a computational model, the predominant secondary metabolites in the essential oil were selected as candidates for interaction analysis with cyclooxygenase-2, an enzyme implicated in the inflammatory response. Our findings suggest that P. leptostachya leaf oil could serve as a potential source of natural compounds with prospective therapeutic effects in treating inflammatory conditions. Full article

In the realm of analysis, the lateral flow immunoassay (LFIA) is frequently utilized due to its capability to be fast and immediate. However, the biggest challenge of the LFIA is its low detection sensitivity and tolerance to matrix interference, making it impossible to enable accurate, qualitative analyses. In this study, we developed a new LFIA with higher affinity and sensitivity, based on a nanobody (G8-DIG) and CuS nanoflowers-Au (CuS NFs-Au), for the detection of aflatoxin B₁ (AFB₁) in maize. We synthesized the immunoprobe G8-DIG@CuS NFs-Au, stimulated the in situ development of Au nanoparticles (Au NPs) on Cu NFs by electrical displacement, and obtained Cu NFs-Au for fixing the G8-DIG. G8-DIG@CuS NFs-Au probe-based LFIAs may, in ideal circumstances, use a strip chromatography reader to accomplish sensitive quantitative detection and qualitative visualization. AFB₁ has a detection range of 2.82–89.56 µg/L and a detection limit of 0.87 µg/L. When compared with an LFIA based on CuS NFs, this sensitivity is increased by 2.76 times. The practical application of this method in corn flour demonstrated a recovery rate of 81.7% to 117%. Therefore, CuS NFs-Au show great potential for detecting analytes. Full article

Bee organisms need nutrients to function properly. Deficiencies of any nutrients decrease the condition and shorten the lifespan of insects. Moreover, protein deficiency decreases honey bee queen productivity and increases aggression in bee colonies. All of these aspects affect the efficiency and the economic aspect of beekeeping production. Limited access to sustainable feed sources for bee colonies during the season forces beekeepers to search for new sources of nutrients, particularly protein. The aim of this study was to investigate the potential use of brewers’ spent grain, which is a by-product of beer production, as a source of protein additive in bees’ diet. Two types of brewers’ spent grain were examined: that from light beer and that from dark porter beer. The spent grains, especially porter spent grains, improved the hemolymph protein content compared to bees fed with sugar cake without additives. It did not fully correspond to the protein levels obtained from bees fed cake with the addition of pollen, but it may be a substitute. The studies showed that brewers’ spent grain has the potential to be used as an alternative plant protein component of honey bee feed. Full article

The circular economy is one of the main strategies for mitigating the environmental impacts of civil construction due to the generation of construction and demolition waste (CDW). In this transition, evaluating alternatives for using buildings as material banks is a way to make the process of reusing construction components more efficient. Thus, the article aimed to evaluate the state of the art of publications on the relationship between the circular economy in civil construction and the conceptual model of buildings as material banks to mitigate the environmental impacts of CDW. The authors chose the methodological design of Systematic Literature Review, using the Scopus and Web of Science databases for research, with the following search strings: (“construction” or “civil construction” or “built environment” or “construction industry”) and (“circular economy” or “circular construction”) and (“material banks” or “BAMB” or “buildings as material banks” or “building stocks” or “building materials”) and (“construction waste” or “demolition waste” or “CDW” or “construction and demolition waste” or “environmental impacts”). After a screening in which only articles published in journals were selected, from 2013 to 2023, inclusion and exclusion criteria were applied, to evaluate only those that had a direct relationship with CDW management through circular economy strategies and buildings such as banks of material. As a result, 93 articles remained, which were analyzed using a quantitative and qualitative approach. The predominance of applied studies was also noted through case studies that evaluate the management of materials and waste in the urban environment. The qualitative analysis, carried out using a SWOT matrix, highlighted the strengths of the buildings, such as material banks, the potential reduction of resource extraction and urban mining, and promoting the circulation of construction products. However, the recycling of waste, such as aggregates, still stands out as the main end-of-life strategy adopted, even without occupying the top of the waste hierarchy. Full article

The COVID-19 pandemic presented an unprecedented challenge to public health as well as an extraordinary burden on health systems worldwide. COVID-19 vaccines were attributed as a key tool to control the pandemic, with healthcare workers (HCWs) as a priority group to receive the vaccine. Healthcare workers are considered one of the most trusted sources of information on vaccines and vaccination. This study was conducted to evaluate the acceptability of the COVID-19 vaccine among HCWs in four different provinces of Indonesia. An anonymous cross-sectional study was conducted online among HCWs between December 2020 and February 2021. Out of 2732 participants, 80.39% stated that they would accept the COVID-19 vaccine, while 19.61% were hesitant to receive the vaccine. Concerns about the safety profile of COVID-19 vaccines and potential side-effects after vaccination were the main reasons among the participants to refuse the vaccine. Male gender, single status, higher education level, and higher risk perception increased the acceptability of the COVID-19 vaccine. Other motivators of COVID-19 vaccine acceptance include a high level of trust in the government and increased confidence in vaccine safety and efficacy studies. Dissemination of information in a timely manner as well as training programs for HCWs are crucial to increasing confidence in the COVID-19 vaccination program. Full article

Based on the characteristics of space particle radiation in the Sun-synchronous orbit (SSO), a space particle radiation effect comprehensive measuring instrument (SPRECMI) was installed on the orbital platform of the upper stage of the Chinese CZ-4C carrier rocket, which can acquire the high-energy proton energy spectra, linear energy transfer (LET) spectra of particles, and radiation dose rate. The particle radiation detection data were obtained at 1000 km altitude for the first time, which can be used mainly for scientific research of the space environment, in-orbit fault analysis, and the operational control management of spacecraft, and can also serve as reference data for component validation tests. After SPRECMI’s development, accelerator calibration and simulations were conducted, and the results demonstrated that all the measured indicators, including the high-energy proton spectra (energy range: 21.8–275.0 MeV, precision: <3.3%), total radiation dose (dose range: 0–1.04 × 10⁶ rad, sensitivity: 6.2 µrad/h), and the LET spectra (range: 0.001–37.20 MeV/(mg/cm²), >37.2 MeV/(mg/cm²)), met the relevant requirements. Furthermore, the in-orbit flight test revealed that the detection results of the load components were consistent with the physical characteristics of the particle radiation environment of the spacecraft’s orbit. Full article