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Sequence and expression of the chicken membrane-associated phospholipases A1 alpha (LIPH) and beta (LIPI)

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Abstract

Cancer/testis antigens (CTA) are a heterogeneous group of antigens that are expressed preferentially in tumor cells and testis. Based on this definition the human membrane-associated phospholipase A1 beta (lipase family member I, LIPI) has been identified as CTA. The high homology of LIPI and the membrane-associated phospholipase A1 alpha (lipase family member H, LIPH) suggests that both genes are derived from a common ancestor by gene duplication. In contrast to human LIPI, human LIPH is expressed in several tissues. LIPI sequences have only been identified in mammals. Here, we describe the identification of LIPI in non-mammalian vertebrates. Based on the conserved genomic organization of LIPI and LIPH we identified sequences for both lipases in birds and fishes. In all vertebrates the LIPI locus is neighbored by a member of the RNA binding motif (RBM) family, RBM11. By sequencing of reverse transcriptase-polymerase chain reaction products we determined the sequences of LIPI and LIPH messenger RNA from broilers. We found that the sequence homology between LIPI and LIPH is much higher in non-mammalian species than in mammals. In addition, we found broad expression of LIPI in broilers, resembling the expression profile of LIPH. Our data suggest that LIPI is a CTA only in mammalian species and that the unique sequence features of the mammalian LIPI/RBM11 locus have evolved together with the CTA-like expression pattern of LIPI.

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Abbreviations

CTA:

Cancer/testis antigens

EFT:

Ewing family tumor

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HD:

Hodgkin’s lymphoma

LCL:

Lymphoblastoid cell lines

LIPH:

Lipase family member H

LIPI:

Lipase family member I

LPA:

Lysophosphatidic acid

NB:

Neuroblastoma

RBM7:

RNA binding motif protein 7

RBM11:

RNA binding motif protein 11

TAA:

Tumor associated antigen

TSA:

Tumor specific antigen

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Acknowledgments

We thank Ines Volkmer and Siggi Heins for grateful technical assistance.

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Authors and Affiliations

  1. Department of Pediatrics, Children’s Cancer Research Centre, Martin-Luther-University Halle-Wittenberg, Ernst Grube Str. 40, 06097, Halle, Germany

    Manuela Hesse, Edith Willscher, Benjamin J. Schmiedel & Martin S. Staege

  2. Institute of Computer Science, Martin-Luther-University Halle-Wittenberg, 06120, Halle, Germany

    Stefan Posch

  3. Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, 06120, Halle, Germany

    Ralph P. Golbik

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  1. Manuela Hesse
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  2. Edith Willscher
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  6. Martin S. Staege
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Correspondence to Martin S. Staege.

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Manuela Hesse and Edith Willscher contributed equally to this work.

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Hesse, M., Willscher, E., Schmiedel, B.J. et al. Sequence and expression of the chicken membrane-associated phospholipases A1 alpha (LIPH) and beta (LIPI). Mol Biol Rep 39, 761–769 (2012). https://doi.org/10.1007/s11033-011-0796-0

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