Abstract
Sialylated or sulfated derivatives and acrylamide copolymers of blood group T-(Galβ1,3GalNAcα-) and Tn-(GalNAcα) haptens were studied for their interaction with the lectins of peanut (PNA),Agaricus bisporus-(ABA),Helix pomatia-(HPA) andVicia villosa B4-(VVA), using asialo Cowper's gland mucin (ACGM), which contains both T and Tn epitopes, as the coating substrate in enzyme linked lectin assay. Both T and Tn copolymers (∼40 haptens) showed high affinity and strict specificity; although the T-copolymer at 0.05–0.07 µm concentration caused 50% inhibition of interaction of either PNA or ABA with ACGM, there was little inhibition of the HPA and VVA interactions at over 100 times that concentration. The Tn-copolymer at 0.02–0.05 µm inhibited HPA or VVA interaction with ACGM by 50% but gave virtually no inhibition of PNA and ABA binding. Sialyl, sulfate or methyl group substitution on C-6 of GalNAc of the T-haptene did not prevent interaction with PNA but almost abolished interaction with ABA. In contrast, sialyl or sulfate group on C-6 and sulfate on C-3 of Gal in Galβ1,3GalNAcα- inhibited almost completely the interaction of PNA with ACGM but had only a slight effect on the interaction of ABA; C-6 substitution with either sialic acid or sulfate on GalNAcα- almost abolished the interaction of both HPA and VVA with ACGM. Preliminary studies revealed a significant depression in the serum level of anti-T (two to three-fold decrease) and anti-Tn (∼ two-fold decrease) antibodies in breast cancer compared with normal control subjects when the acrylamide T- and Tn-copolymers were used as coating substrates in enzyme linked immunoassays.
Similar content being viewed by others
Abbreviations
- PNA:
-
peanut agglutinin
- ABA:
-
agaricus bisporus agglutinin
- HPA:
-
helix pomatia agglutinin
- VVA:
-
(B4),vicia villosa agglutinin
- ACGM:
-
Asialo Copwer's gland mucin
- CA:
-
carcinoma
- BSA:
-
bovine serum albumin
- HRP:
-
Horseradish peroxidase
- ABTS:
-
2,2′-azino-di (3-ethyl-benzthiazoline sulfonate)
- ELISA:
-
enzyme-linked immunosorbent assay
- Al:
-
allyl
- Bn:
-
benzyl
- AA:
-
acrylamide
- CP:
-
copolymer
References
Springer GF (1984)Science 224:1198–1206.
Inoue M, Nakada H, Tanaka N, Yamashina I. (1994)Cancer Res 54:85–88.
Hamada S, Furumoto H, Kamada M, Hirao T, Aono T (1993)Cancer Lett 74:167–173.
Hirao T, Sakamoto Y, Kamada M, Hamada S, Aono T (1993)Cancer 72:154–59.
Therkildsen MH, Manokl U, Christensen M, Dabelsteen E (1993)Cancer 72:1147–54.
Ghazizadeh M, Oguro T, Sasaki Y, Aihara K, Springer GF (1990)Am J Clin Pathol 93:315–21.
Blasco E, Torrado J, Belloso L, Arocena F, Gutierrez-Hoyos A, Cuadrado E (1988)Cancer 61:1091–95.
Fenlon S, Ellis IO, Bell J, Todd JH, Elston CW, Blarney RW (1987)J Pathol 152:169–76.
Leathem AJ, Brooks SA (1987)Lancet i, 1054–56.
Springer GF (1989)Molec Immun 26:1–5.
Wu AM, Sugii S (1991)Carbohydr Res 213:127–43.
Knibbs RN, Goldstein IJ, Ratcliffe RM, Shibuya N (1991)J Biol Chem 266:83–88.
O'Beirne AJ, Cooper HR (1979)J Histochem Cytochem 27:1148–62.
Chandrasekaran EV, Jain RK, Matta KL (1992)J Biol Chem 267:19929–37.
Horejsi V, Smolek P, Kocowrek J (1978)Biochim Biophys Acta 538:293–98.
Yazawa S, Madiyalakan R, Chawda RP, Matta KL (1986)Biochem Biophys Res Commun 136:563–69.
DeVries AL, Komatsu SK, Feeney RE (1970)J Biol Chem 245:2901–8.
Komatsu SK, DeVries AL, Feeney RE (1970)J Biol Chem 245:2909–13.
Jain RK, Piskorz CF, Matta KL (1993)Carbohydr Res 243:385–91.
Matta KL, Piskorz CF, Reddy GV, Chandrasekaran EV, Jain RK (1994) ACS Symposium Series 560 Kovac P (Ed.) pp. 120–32.
Lotan R, Skutelsky E, Damon D, Sharon N (1975)J Biol Chem 250:8518–23.
Pereira MEA, Kabat EA, Lotan R, Sharon N (1976)Carbohydr Res 51:107–18.
King M-E, Holburn AM (1979)Immunology 38:129–36.
Kaifu R, Osawa T (1979)Carbohydr Res 69:79–88.
Presant CA, Kornfeld S (1972)J Biol Chem 247:6937–45.
Hammarstrom S, Kabat EA (1971)Biochemistry 10:1684–92.
Tollefsen SE Kornfeld R (1983)J Biol Chem 258:5165–71.
Yamori T, Kimura H, Stewart K, Ota DM, Cleary KR, Irimura T (1987)Cancer Res 47:2741–47.
Mawhinney TP, Landrum DC, Gayer DA, Barbero GJ (1992)Carbohydr Res 235:179–97.
Springer GF, Taylor CR, Howard DR, Tegtmeyer H, Desai PR, Murthy SM, Felder B, Scanlon EF (1985)Cancer 55:561–69.
Toyokuni T, Dean B, Cai S, Boivin D, Hakomori S, Singhal AK (1994)J Am Chem Soc 116:395–96.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, Y., Jain, R.K., Chandrasekaran, E.V. et al. Use of sialylated or sulfated derivatives and acrylamide copolymers of Galβ1,3GalNAcα- and GalNAcα- to determine the specificities of blood group T- and Tn-specific lectins and the copolymers to measure anti-T and anti-Tn antibody levels in cancer patients. Glycoconjugate J 12, 55–62 (1995). https://doi.org/10.1007/BF00731869
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00731869