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Transformation of triclosan by a novel cold-adapted laccase from Botrytis sp. FQ

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Abstract

This work investigated the transformation of triclosan (TCS) by the laccase produced by a pathogen isolated from rotten tomato. The pathogen was characterized as Botrytis sp. FQ, belonging to subphylum Deuteromycotina. The laccase exhibited cold-adaptation with relatively high activity at 20°C. The laccase could effectively transform TCS. Approximately 62% TCS could be removed at dose of 1.0 unit∙mL–1 in 120 min. The reaction rate appeared to be pseudo-first-order to the concentration of the substrate, suggesting the laccase activity remained stable during the reaction. Transformation products of TCS were analyzed by mass spectrometry and it was revealed that TCS dimers were formed via radical coupling pathways. During this process, laccase catalyzed oxidation of TCS to form a radical intermediate is the rate limiting step. However, this step can be reversed by humic acid. Overall, the laccase showed great potential in the treatment of phenolic contaminants. Since laccase is widely presented in natural environment, this study also revealed an important pathway involved in the transformation of phenolic contaminants in the environment.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (Grant No. 51503074), the Fundamental Research Funds for Central Universities (KYZ201626) and the priority Academic Program Development (PAPD) of Jiangsu Higher Education Institute. We would like to thank Kang Wu (SEM laboratory, Jiangnan University, Wuxi, China) for his assistance in SEM observation.

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Authors and Affiliations

  1. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing, 210095, China

    Yuanyuan Shi, Junhe Lu, Xiaoming Yin & Quansuo Zhou

  2. Nanjing Institute of Environmental Science, Ministry of Environmental Protection of PRC, Nanjing, 210042, China

    Deyang Kong

  3. School of Bioengineering, Huanghuai University, Zhumadian, 463000, China

    Jiayang Liu

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  1. Yuanyuan Shi
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  2. Deyang Kong
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  3. Jiayang Liu
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  4. Junhe Lu
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  5. Xiaoming Yin
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Correspondence to Jiayang Liu or Junhe Lu.

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Shi, Y., Kong, D., Liu, J. et al. Transformation of triclosan by a novel cold-adapted laccase from Botrytis sp. FQ. Front. Environ. Sci. Eng. 11, 6 (2017). https://doi.org/10.1007/s11783-017-0927-5

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  • DOI: https://doi.org/10.1007/s11783-017-0927-5

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