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Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology

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Abstract

We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5′-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with 6-formylbenzo[a]pyrene. So, this SERS detection involves four steps: (i) the nanoprobes are incubated with the sample; (ii) a drop of the mixture is then put onto the substrate; (iii) the surface is rinsed; and (iv) the surface is analyzed by Raman spectroscopy. To synthesize the nanoprobes, firstly, we prepared GNPs according to Frens’ method. Then, GNPs were spontaneously labeled by the DSNB Raman reporter, thanks to a strong gold-sulfur interaction. Thereafter, BaP antibodies were cross-linked to the DSNB labeled GNPs by reaction of proteins primary amino groups with N-hydroxyl succinimide (NHS). Before use in SERS detection, their activity was controlled by surface plasmon resonance technique. The present method allows us to detect BaP at trace concentration (2 nmol/L). The results demonstrate that the proposed method has a great potential for application in the monitoring of seawater.

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Acknowledgments

This project was financing by ANR, the French Research Agency thanks to the Ifremer-Edrome Carnot Institute Grants and thanks to the ECOTECH 2011 program, through the ANR-11-ECOT-010-REMANTAS project. Many thanks to Morgan Tardivel for the proofreading.

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Authors and Affiliations

  1. IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France

    Mohamed Dribek, Emmanuel Rinnert, Florent Colas, Marie-Pierre Crassous & Chantal Compère

  2. Laboratoire CSPBAT (FRE 3043), UFR SMBH, Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France

    Néné Thioune, Catalina David & Marc de la Chapelle

  3. EA 6295 Nanomédicaments et Nanosondes, UFR des Sciences Pharmaceutiques, Université François-Rabelais de Tours, 31 avenue Monge, 37200, Tours, France

    Mohamed Dribek

  4. Horiba Scientific, 231 rue de, Lille, 59650, Villeneuve d′Ascq, France

    Catalina David

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  1. Mohamed Dribek
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  2. Emmanuel Rinnert
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  3. Florent Colas
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  8. Chantal Compère
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Correspondence to Emmanuel Rinnert.

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Responsible editor: Philippe Garrigues

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Dribek, M., Rinnert, E., Colas, F. et al. Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology. Environ Sci Pollut Res 24, 27070–27076 (2017). https://doi.org/10.1007/s11356-014-3384-8

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  • DOI: https://doi.org/10.1007/s11356-014-3384-8

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