Skip to main content
SpringerLink
Log in

Genome Characterization of Pseudomonas aeruginosa KT1115, a High Di-rhamnolipid-Producing Strain with Strong Oils Metabolizing Ability

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

In this study, a wild-type Pseudomonas aeruginosa strain KT1115 with the capability of converting rapeseed oils into di-rhamnolipids, a class of biosurfactants with extensive application potential, was successfully isolated and characterized. Di-rhamnolipids production by microorganism culture provided a mild, eco-friendly, and secure approach for surfactants production instead of conventional chemical synthesis. However, few studies have been attempted to explore the metabolic mechanism behind the high di-rhamnolipids production by P. aeruginosa. Here, we presented the graft genome of a wild-type P. aeruginosa strain KT1115, with emphasis on the analysis of oils metabolism and rhamnolipid synthesis. The availability of the genome sequence provides additional insight into the genetic mechanism enhancing di-rhamnolipids biosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Mulligan C, Yong R, Gibbs B (2001) Surfactant-enhanced remediation of contaminated soil: a review. Eng Geol 60:371–380

    Article  Google Scholar 

  2. Henkel M, Müller MM, Kügler JH, Lovaglio RB, Contiero J, Syldatk C, Hausmann R (2012) Rhamnolipids as biosurfactants from renewable resources: concepts for next-generation rhamnolipid production. Process Biochem 47:1207–1219

    Article  CAS  Google Scholar 

  3. Wadekar S, Kale S, Lali A, Bhowmick D, Pratap A (2012) Microbial synthesis of rhamnolipids by Pseudomonas aeruginosa (ATCC 10145) on waste frying oil as low cost carbon source. Prep Biochem Biotechnol 42:249–266

    Article  CAS  Google Scholar 

  4. Tan YN, Li Q (2018) Microbial production of rhamnolipids using sugars as carbon sources. Microb Cell Factories 17:89

    Article  Google Scholar 

  5. Muller MM, Hormann B, Syldatk C, Hausmann R (2010) Pseudomonas aeruginosa PAO1 as a model for rhamnolipid production in bioreactor systems. Appl Microbiol Biotechnol 87:167–174

    Article  Google Scholar 

  6. Jie Z, Xue R, Liu S, Xu N, Xin F, Zhang W, Jiang M, Dong W (2019) High di-rhamnolipid production using Pseudomonas aeruginosa KT1115, separation of mono/di-rhamnolipids, and evaluation of their properties. Front Bioeng Biotechnol 7:245

    Article  Google Scholar 

  7. Lopez G, Diaz-Cardenas C, Shapiro N, Woyke T, Kyrpides NC, David Alzate J, Gonzalez LN, Restrepo S, Baena S (2017) Draft genome sequence of Pseudomonas extremaustralis strain USBA-GBX 515 isolated from Superparamo soil samples in Colombian Andes. Stand Genomic Sci 12:78

    Article  Google Scholar 

  8. Schubert M, Lindgreen S, Orlando L (2016) AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Res Notes 9:88

    Article  Google Scholar 

  9. Khan AL, Asaf S, Khan AR, Al-Harrasi A, Al-Rawahi A, Lee I-J (2016) First draft genome sequencing of indole acetic acid producing and plant growth promoting fungus Preussia sp. BSL10. J Biotechnol 225:44–45

    Article  CAS  Google Scholar 

  10. Sun C, Li Y, Wu Q, Luo H, Sun Y, Song J, Lui EM, Chen S (2010) De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis. BMC Genomics 11:262

    Article  Google Scholar 

  11. Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955

    Article  CAS  Google Scholar 

  12. Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O (1994) Bacterial lipases. FEMS Microbiol Rev 15:29–63

    Article  CAS  Google Scholar 

  13. Dobler L, Vilela LF, Almeida RV, Neves BC (2016) Rhamnolipids in perspective: gene regulatory pathways, metabolic engineering, production and technological forecasting. N Biotechnol 33:123–135

    Article  CAS  Google Scholar 

  14. Deziel E, Lepine F, Milot S, Villemur R (2003) rhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs), the precursors of rhamnolipids. Microbiology 149:2005–2013

    Article  CAS  Google Scholar 

  15. Rahim R, Ochsner UA, Olvera C, Graninger M, Messner P, Lam JS, Soberón-Chávez G (2001) Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis. Mol Microbiol 40:708–718

    Article  CAS  Google Scholar 

  16. Winsor GL, Lam DK, Fleming L, Lo R, Whiteside MD, Yu NY, Hancock RE, Brinkman FS (2010) Pseudomonas Genome Database: improved comparative analysis and population genomics capability for Pseudomonas genomes. Nucleic Acids Res 39:D596–D600

    Article  Google Scholar 

  17. Abdel-Mawgoud AM, Lépine F, Déziel E (2014) A stereospecific pathway diverts β-oxidation intermediates to the biosynthesis of rhamnolipid biosurfactants. Chem Biol 21:156–164

    Article  CAS  Google Scholar 

  18. Raychaudhuri A, Tullock A, Tipton PA (2008) Reactivity and reaction order in acylhomoserine lactone formation by Pseudomonas aeruginosa RhlI. Biochemistry 47:2893–2898

    Article  CAS  Google Scholar 

  19. Kambam PKR, Eriksen DT, Lajoie J, Sayut DJ, Sun L (2009) Altering the substrate specificity of RhlI by directed evolution. ChemBioChem 10:553–558

    Article  CAS  Google Scholar 

  20. Rosenau F, Jaeger K-E (2000) Bacterial lipases from Pseudomonas: regulation of gene expression and mechanisms of secretion. Biochimie 82(11):1023–1032

    Article  CAS  Google Scholar 

  21. Zhang B, Ren L, Xu D, Wang H, Chen Z, Zhang B, Zeng X, Sun L, Li F (2020) Directed evolution of RhlI to generate new and increased quorum sensing signal molecule catalytic activities. Enzyme Microb Technol 134:109475

    Article  CAS  Google Scholar 

  22. Moretti R, Chang A, Peltier-Pain P, Bingman CA, Phillips GN Jr, Thorson JS (2011) Expanding the nucleotide and sugar 1-phosphate promiscuity of nucleotidyltransferase RmlA via directed evolution. J Biol Chem 286:13235–13243

    Article  CAS  Google Scholar 

  23. Reis RS, Pereira AG, Neves BC, Freire DMG (2011) Gene regulation of rhamnolipid production in Pseudomonas aeruginosa—a review. Bioresour Technol 102:6377–6384

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Key R&D Program of China (No. 2018YFA0902200), National Natural Science Foundation of China (Nos. 31961133017, 31700092, 21978129), the Natural Science Foundation of Jiangsu Province (No. BK20170997), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture, Jiangsu Agriculture Science and Technology Innovation Fund [NO. CX (19)3104], Project of State Key Laboratory of Materials-Oriented Chemical Engineering (No. ZK201601).

Author information

Author notes

    Authors and Affiliations

    1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People’s Republic of China

      Shixun Liu, Ning Xu, Haojie Liu, Jie Zhou, Fengxue Xin, Wenming Zhang, Xiujuan Qian, Min Jiang & Weiliang Dong

    2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People’s Republic of China

      Jie Zhou, Fengxue Xin, Wenming Zhang, Min Jiang & Weiliang Dong

    Authors
    1. Shixun Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Ning Xu
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Haojie Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Jie Zhou
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Fengxue Xin
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Wenming Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Xiujuan Qian
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Min Jiang
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Weiliang Dong
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Weiliang Dong.

    Ethics declarations

    Conflict of interest

    The authors have declared that they have no conflict of interest.

    Additional information

    Publisher's Note

    Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

    Nucleotide Sequence Accession Numbers

    This Whole Genome project has been deposited into GenBank database under the Accession Number WUWO00000000. The chromosome is under the accession of MK386864.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Liu, S., Xu, N., Liu, H. et al. Genome Characterization of Pseudomonas aeruginosa KT1115, a High Di-rhamnolipid-Producing Strain with Strong Oils Metabolizing Ability. Curr Microbiol 77, 1890–1895 (2020). https://doi.org/10.1007/s00284-020-02009-z

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00284-020-02009-z

    Search

    Navigation