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Capacity factors and peak widths in the gradient elution reversed phase separation of high molar mass polystyrenes

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Summary

A simplex method for determining the constantsS andk o from the equation lnk’=lnk o−S ϕ (was developed and applied to the reversed phase separation of high molar mass polystyrenes using gradients of any curvature. Experimental retention times described using the equation had a standard deviation of 1.1%. The inclusion of a quadratic term in the equation was found to be unwarranted. BothS and lnk o varied linearly with ln molar mass. The logarithm of peak width of an eluting polystyrene peak was found to be a linear function of the mobile phase composition at elution. The slope was equal toS.

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Authors and Affiliations

  1. School of Biological and Chemical Sciences, Deakin University, Geelong, 3217, Victoria, Australia

    T. L. Pang & P. E. Kavanagh

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  1. T. L. Pang
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  2. P. E. Kavanagh
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Pang, T.L., Kavanagh, P.E. Capacity factors and peak widths in the gradient elution reversed phase separation of high molar mass polystyrenes. Chromatographia 46, 12–16 (1997). https://doi.org/10.1007/BF02490924

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