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Purification and properties of a heat-resistant exotoxin produced byMacrophomina phaseolina (Tassi) Goid in culture

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Summary

A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain ofMacrophomina phaseolina, isolated fromPhaseolus mungo L. could reduce Cu++ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cut seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings ofP. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase.

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Authors and Affiliations

  1. Microbiological Laboratory, Department of Botany, University of Burdwan, 713104, Burdwan, India

    K. A. I. Siddiqui, A. K. Gupta, A. K. Paul & A. K. Banerjee

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  1. K. A. I. Siddiqui
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  2. A. K. Gupta
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  3. A. K. Paul
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  4. A. K. Banerjee
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Siddiqui, K.A.I., Gupta, A.K., Paul, A.K. et al. Purification and properties of a heat-resistant exotoxin produced byMacrophomina phaseolina (Tassi) Goid in culture. Experientia 35, 1222–1223 (1979). https://doi.org/10.1007/BF01963302

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