Abstract
Changes in microbial activities and bacterial community composition (BCC) were studied using fluorescence in situ hybridization (FISH) along the longitudinal axis of two canyon-shaped reservoirs with contrasting retention times and nutrient and organic matter loads. The reservoirs showed different specific longitudinal changes in plankton communities and BCC shifts that were related mainly to allochthonous input and longitudinal patterns of primary production and nutrient availability. The studied branch of the Orlík reservoir is entered by a large river and so had a short retention time (23 days) and a large allochthonous input. Primary production, bacterial abundance, bacterial production and protozoan bacterivory showed a consistent trend, decreasing gradually from the river inflow downstream. Overall, shifts in BCC were minor and statistically insignificant. The relatively large input of the river-borne allochthonous plankton communities and the short retention time of the reservoir probably limited the effect of the autochthonous in-reservoirs processes. In contrast, the Římov reservoir has a long retention time (∼100 days) and had a limited input of allochthonous organic matter to its lacustrine area. The autochthonous processes of primary and bacterial production were tightly linked and they showed peaks in the middle part of the studied stretch. This together with a trend of downstream decreasing nutrient concentrations and increasing protistan grazing pressure seem to support the development of specific BCC in the lacustrine part of the reservoir that significantly differed from that in river inflow. Apart from the marked differences between the limnological variables of reservoirs we found some commonalties in BCC – in both systems the phylogenetic groups of β-Proteobacteria and Cythophaga/Flavobacterium clearly dominated bacterioplankton assemblages. %
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Alfreider, A., J. Pernthaler, R. I. Amann, B. Sattler, F. O. Glöckner, A. Ville R. Psenner, 1996. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake using in situ hybridization. Appl. environ. Microbiol. 62: 2138–2144.
Amann, R.I., 1995. Fluorescently labelled, rRNA-targeted oligonucleotide probes in the study of microbial ecology. Microb. Ecol. 4: 543–554.
Armengol, J., J. C. Garcia, M. Comerma, M. Romero, J. Dolz, M. Roura, B. P. Han, A. Vidal K. Šimek, 1999. Longitudinal processes in canyon type reservoirs: The case of Sau (N.E. Spain). In Tundisi, J. G. M. Straškraba (eds), Theoretical Reservoir Ecology and its Applications. Brazilian Academy of Sciences and Backhuys Publishers: 313–345.
Azam, F., T. Fenchel, J. G. Field, J. S. Gray, L. A. Meyerreil F. Thingstad, 1983. The ecological role of water-column microbes in the sea. Mar. Ecol. Prog. Ser. 10: 257–263.
Borovec, J. J. Hejzlar, 2001. Phosphorus fractions and phosphorus sorption characteristics of freshwater sediments an their relationship to sediment composition. Arch. Hydrobiol. 151: 687–703.
Bouvier, T. C. P. A. delGiorgio, 2002. Compositional changes in free-living bacterial communities along a salinity gradient in two temperare estuaries. Limnol. Oceanogr. 47: 453–470.
Chrzanowski, T. H., R. W. Sterner J. J. Elser, 1995. Nutrient enrichment and nutrient regeneration stimulate bacterioplankton growth. Microb. Ecol. 29: 221–230.
Comerma, M., C. J. García, J. Armengol, M. Romero K. Šimek, 2001. Planktonic food web structure along the Sau Reservoir (Spain) in summer 1997. Int. Rev. Hydrobiol. 86: 195–209.
Daims, H., A. Brühl, R. Amann, K. H. Schleifer M. Wagner, 1999. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Syst. appl. Microbiol. 22: 434–444.
delGiorgio, P. A., J. M. Gasol., D. Vaque, P. Mura, S. Agusti C. M. Duarte, 1996. Bacterioplankton community structure: Protists control net production and the proportion of active bacteria in a coastal marine community. Limnol. Oceanogr. 41: 1169–1179.
Frischer, M. E., P. J. Floriani S. A. Nierzwicki-Bauer, 1996. Differential sensitivity of 16S rRNA targeted oligonucleotide probes used for fluorescence in situ hybridization is a result of ribosomal higher order structure. Can. J. Microbiol. 42: 1061–1071.
Fuhrman, J. A., 1999. Marine viruses and their biogeochemical and ecological effects. Nature 399: 541–548.
Gasol, J. M., M. Comerma, J. C. Garcia, J. Armengol, E. O. Casamayor, P. Kojecká K. Šimek, 2002. A transplant experiment to identify the factors controlling bacterial abundance, activity, production, and community composition in a eutrophic canyon-shaped reservoir. Limnol. Oceanogr. 47: 62–77.
Glöckner, F. O., B. M. Fuchs R. Amann, 1999. Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl. environ. Microbiol. 65: 3721–3726.
Glöckner, F. O., E. Zaichikov, N. Belkova, L. Denissova, J. Pernthaler, A. Pernthaler R. Amann, 2000. Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phyllogenetic clusters including an abundant group of actinobacteria. Appl. environ. Microbiol. 66: 5053–5065.
Hahn, M. W. M. G. Höfle, 1998. Grazing pressure by a bacterivorous flagellate reverses the relative abundance of Comamonas acidovorans PX 54 and Vibrio strain CB5 in chemostat cocultures. Appl. environ. Microbiol. 64: 1910–1918.
Hahn, M. W. M. G. Höfle, 2001. Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microb. Ecol. 35: 113–121.
Hejzlar, J. V. Vyhnálek, 1998. Longitudinal heterogeneity of phosphorus and phytoplankton concentrations in deep-valley reservoirs. Int. Rev. Hydrobiol. 83 (Special issue): 139–146.
Hicks, R. E., R. I. Amann & D. A. Stahl, 1992. Dual staining of natural bacterioplankton with 4′,6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom – level 16S rRNA sequences. Appl. environ. Microbiol. 58: 2158–2163.
Jezbera, J., J. Nedoma K. Šimek, 2003. Longitudinal changes in protistan bacterivory and bacterial production in two canyonshaped reservoirs of different trophic status. Hydrobiologia 504: 115–130.
Jürgens, K., 1994. Impact of Daphnia on planktonic microbial food webs. A review. Mar. Microb. Food Webs 8: 295–324.
Jürgens, K., J. Pernthaler, S. Schalla R. Amann, 1999. Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing. Appl. environ. Microbiol. 65: 1241–1250.
Kalff, J., 2002. Limnology: Inland water ecosystems. Prentice-Hall Inc., Upper Saddle River, NJ, 592 pp.
Leff, L. G., 2000. Longitudinal changes in microbial assemblages of the Ogeechee River. Freshwat. Biol. 43: 605–616.
Linsdtröm, E. S., 2000. Bacterioplankton community composition in five lakes differing in trophic status and humic content. Microb. Ecol. 40: 104–113.
Lorenzen, C. J., 1967. Determination of chlorophyll and phaeopigments. Spectrophotometric equations. Limnol. Oceanogr. 12: 343–346.
Manz, W., K. Wendt-Ponthoff, T. R. Neu, U. Szewzyk J. R. Lawrence, 1999. Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and confocal laser scanning microscopy. Microb. Ecol. 37: 225–237.
Methé, B. A., W. D. Hiorns J. P. Zehr, 1998. Contrasts between marine and freshwater bacterial community composition: Analyses of communities in Lake George and six other Adirondack lakes. Limnol. Oceanogr. 43: 368–374.
Murphy, I. J. P. Riley, 1962. A modified single-solution method for determination of phosphate in natural waters. Anal. Chim. Acta 27: 31–36.
Pace, M. L. J. J. Cole, 1994. Comparative and experimental approaches to top-down and bottom-up regulation of bacteria. Microb. Ecol. 28: 181–193.
Pernthaler, J., A. Alfreider, T. Posch, S. Andreatta R. Psenner, 1997. In situ classification and image cytometry of pelagic bacteria from a high mountain lake (Gossenkollesee, Austria). Appl. environ. Microbiol. 63: 4778–4783.
Pernthaler, J., F. O. Glöckner, S. Unterholzner, A. Alfreider, R. Psenner R. Amann, 1998. Seasonal community and population dynamics of pelagic Bacteria and Archaea in a high mountain lake. Appl. environ. Microbiol. 64: 4299–4306.
Pernthaler, J., T. Posch, K. Šimek, J. Vrba, A. Pernthaler, F. O. Glöckner, U. Nubel, R. Psenner R. Amann, 2001. Predator-specific enrichment of actinobacteria from a cosmopolitan freshwater clade in mixed continuous culture. Appl. environ. Microbiol. 67: 2145–2155.
Pinel-Alloul, B., 1995. Spatial heterogeneity as a multiscale characteristic of a zooplankton community. Hydrobiologia 300/301: 17–42.
Pomeroy, L. R., 1974. The ocean's food web, a changing paradigm. Bioscience 24: 499–504.
Poulsen, L. K., G. Ballard D. A. Stahl, 1993. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. environ. Microbiol. 59: 1354–1360.
Riemann, B. M. Sondergaard, 1986. Regulation of bacterial secondary production in two eutrophic lakes and in experimental enclosures. J. Plankton Res. 8: 519–536.
Roberts, E. C., J. Laybourn-Parry, D. M. McKnight G. Novarino, 2000. Stratification and dynamics of microbial loop communities in Lake Fryxell, Antarctica. Freshwat. Biol. 44: 649–661.
Schindler, D.W., 1977. Evolution of phosphorus limitation in lakes. Science 195: 260–262.
Schauer, M., R. Massana C. Pedrós-Alió, 2000. Spatial differences in bacterioplankton composition along the Catalan coast (NW Mediterranean) assessed by molecular finger printing. FEMS Microb. Ecol. 33: 51–59.
Schweitzer, B. M. Simon, 1995. Growth limitation of planktonic bakteria in a large mesotrophic lake. Microb. Ecol. 30: 89–104.
Shapiro, J., 1988. Introductory Lecture at the International Symposium 'Phosphorus in Freshwater Ecosystems, Uppsala, Sweden', October 1985. Hydrobiologia 179: 9–17.
Sherr, B. F., E. B. Sherr J. McDaniel, 1992. Effect of protistan grazing on the frequency of dividing cells in bacterioplankton assemblage. Appl. environ. Microbiol. 58: 2381–2385.
Sherr, E. B. B. F. Sherr, 1993. Protistan grazing rates via uptake of fluorescently labeled prey. In Kemp, P. F., B. F. Sherr, E. B. Sherr J. J. Cole (eds), Handbook of Methods in Aquatic Microbial Ecology. Lewis Publishers, Boca Raton, FL: 695–701.
Šimek, K., M. Macek, J. Sed'a V. Vyhnálek, 1990. Possible food chain relationships between bacterioplankton, protozoans, and cladocerans in a Reservoir. Int. Rev. Hydrobiol. 75: 583–596.
Šimek, K., J. Bobková, M. Macek, J. Nedoma R. Psenner, 1995. Ciliate grazing on picoplankton in a eutrophic reservoir during the summer phytoplankton maximum: a study at the species and community level. Limnol. Oceanogr. 40: 1077–1090.
Šimek, K., J. Vrba, J. Pernthaler, T. Posch, P. Hartman, J. Nedoma R. Psenner, 1997. Morphological and genotypic shifts in an experimental bacterial community influenced by protists of contrasting feeding modes. Appl. environ. Microbiol. 63: 587–595.
Šimek, K., J. Armengol, M. Comerma, J. C. Garcia, T. H. Chrzanowski, M. Macek, J. Nedoma V. Straškrabová, 1998. Characteristics of protistan control of bacterial production in three reservoirs of different trophy. Int. Rev. Hydrobiol. 83: 485–494.
Šimek, K., J. Armengol, M. Comerma, J. C. Garcia, T. H. Chrzanowski, P. Kojecká, M. Macek, J. Nedoma V. Straškrabová, 1999a. Impacts of protistan grazing on bacterial dynamics and composition in reservoirs of different trophy. In Tundisi, J. G. M. Straškraba (eds), Theoretical Reservoir Ecology and its Applications. Brazilian Academy of Sciences Backhuys Publishers: 267–282.
Šimek, K., P. Kojecká, J. Nedoma, P. Hartman, J. Vrba J. R. Dolan, 1999b. Shifts in bacterial community composition associated with different microzooplankton size fractions in a eutrophic reservoir. Limnol. Oceanogr. 44: 1634–1644.
Šimek, K., J. Armengol, M. Comerma, J. C. Garcia, P. Kojecká, J. Nedoma J. Hejzlar, 2001a. Changes in the epilimnetic bacterial community composition, production, and protist-induced mortality along the longitudinal axis of a highly eutrophic reservoir. Microb. Ecol. 42: 359–371.
Šimek, K., J. Pernthaler, M. G. Weinbauer, K. Horák, J. R. Dolan, J. Nedoma, M. Mašín R. Amann, 2001b. Changes in bacterial community composition and dynamics and viral mortality rates associated with enhanced flagellate grazing in a mesotrophic reservoir. Appl. environ. Microbiol. 67: 2723–2733.
Straškraba, M., 1998. Limnological differences between deep valley reservoirs and deep lakes. Int. Rev. Hydrobiol. (Spec Issue) 83: 1–12.
Straškrabová, V., 1983. The effect of substrate shock on populations of starving aquatic bacteria. J. Appl. Bacteriol. 54: 217–224.
Straškrabová, V., C. Callieri, P. Carrillo, L. Cruz-Pizarro, J. Fott, P. Hartman, M. Macek, J. P. Medina-Sánchez, J. Nedoma K. Šimek, 1999. Investigation on pelagic food webs in mountain lakes – aims and methods. J. Limnol. 58: 77–87.
Thingstad, T. F. R. Lignell, 1997. Theoretical models for the control of bacterial growth rate, abundance, diversity and carbon demand. Aquat. Microb. Ecol. 13: 19–27.
Thornton, K. W., B. L. Kimmel F. F. Payne, 1990. Reservoir Limnology: Ecological Perspectives. Wiley and Sons, New York: 246 pp.
Urabe, J., 1989. Relative importance of temporal and spatial heterogeneity in zooplankton community of an artificial reservoir. Hydrobiologia 184: 1–6.
Weiss, P., B. Schweitzer, R. Amann M. Simon, 1996. Identification in situ and dynamics of bacteria on limnetic organic aggregates. Appl. environ. Microbiol. 62: 1998–2005.
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Mašín, M., Jezbera, J., Nedoma, J. et al. Changes in bacterial community composition and microbial activities along the longitudinal axis of two canyon-shaped reservoirs with different inflow loading. Hydrobiologia 504, 99–113 (2003). https://doi.org/10.1023/B:HYDR.0000008512.04563.0b
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DOI: https://doi.org/10.1023/B:HYDR.0000008512.04563.0b