biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:105-112, 2016 | DOI: 10.1007/s10535-015-0567-1

Identification of a drought responsive gene encoding a nuclear protein involved in drought and freezing stress tolerance in Arabidopsis

H. -D. Moon1, M. -S. Lee1, S. -H. Kim1, W. -J. Jeong2, D. -W. Choi1,*
1 Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University, Gwangju, Korea
2 Korea Research Institute of Biosciences and Biotechnology, Yusong, Daejon, Korea

Plants have developed adaptive strategies to survive under different abiotic stressors. To identify new components involved in abiotic stress tolerance, we screened unannotated expressed sequence tags (ESTs) and evaluated their cold or drought response in Arabidopsis. We identified a drought response gene (DRG) encoding a 39.5-kDa polypeptide. This protein was expressed specifically in siliques and was induced by drought stress in most tissues. When a DRG-GFP construct was introduced into Arabidopsis protoplasts, GFP signals were detected only in the nucleus. The drg mutant plant was more sensitive to mannitol-induced osmotic stress in agar plates and to drought or freezing stress in soil than the wild-type. Activating the DRG restored the normal sensitivity of drg mutants to abiotic stressors. No differences in drought or freezing tolerance were observed between the wild-type and transgenic plants overexpressing the DRG. When DRG was expressed in a cold-sensitive Escherichia coli strain BX04, the transformed bacteria grew faster than the untransformed BXO4 cells under cold stress. These results demonstrate that DRG is a nuclear protein induced by abiotic stresses and it is required for drought and freezing tolerance in Arabidopsis.

Keywords: DRG; drg knock out mutant; Escherichia coli; mannitol; transgenic plants
Subjects: drought-responsive genes; drought tolerance; freezing tolerance; proteins; knock out mutant; mannitol; transgenic plants

Received: January 22, 2015; Revised: May 29, 2015; Accepted: July 27, 2015; Published: January 1, 2016  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Moon, H.-D., Lee, M.-S., Kim, S.-H., Jeong, W.-J., & Choi, D.-W. (2016). Identification of a drought responsive gene encoding a nuclear protein involved in drought and freezing stress tolerance in Arabidopsis. Biologia plantarum, 60(1), 105-112. doi: 10.1007/s10535-015-0567-1
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download filebpl-201601-0011_S1.pdf

    File size: 239.21 kB

    References

    1. Artus, N.N., Uemura, M., Steponkus, P.L., Gilmour, S.J., Lin, C., Thomashow, M.F.: Constitutive expression of the coldregulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance. - Proc. nat. Acad. Sci. USA 93: 13404-13409, 1996. Go to original source...
    2. Bray, E.A., Bailey-Serres, J., Weretilnyk, E.: Responses to abiotic stresses. - In Buchanan, B., Gruissem, W., Jones, R. (ed.): Biochemistry and Molecular Biology of Plants. Pp. 1158-1203, Amer. Soc. Plant Biologist, Rockville 2000.
    3. Choi, H., Hong, J., Ha, J., Kang, J., Kim, S.Y.: ABFs, a family of ABA-responsive element binding factors. - J. biol. Chem. 275: 1723-1730, 2000. Go to original source...
    4. Choi, S., Hwang, M.S., Im, S.O., Kim, N.J., Jeong, W.J., Park, E.J., Gon, Y.G., Choi, D.W.: Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high-temperature condition. - J. appl. Phycol. 25:1237-1246, 2013. Go to original source...
    5. Close, T.J.: Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. - Physiol. Plant 97; 795-803, 1996. Go to original source...
    6. Cuming, A.C., Cho, S.H., Kamisugi, Y., Graham, H., Quatrano, R.S.: Microarray analysis of transcriptional responses to abscisic acid and osmotic, salt, and drought stress in the moss, Physcomitrella patens. - New Phytol. 176: 275-287, 2007. Go to original source...
    7. Diatchenko, L., Lau, Y.C., Campbell, A.P., Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdiov, E.D., Siebert, P.D.: Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. - Proc. nat. Acad. Sci. USA 93: 6025-6030, 1996. Go to original source...
    8. Ha, Y.I., Lim, J.M., Ko, S.M., Liu, J.R., Choi, D.W.: A ginseng-specific abundant protein (GSAP) located on the cell wall is involved in abiotic stress tolerance. - Gene 386:115-122, 2007. Go to original source...
    9. Hirayama, T., Shinozaki, K.: Research on plant abiotic stress responses in the post-genome era: past, present and future. - Plant J. 61:1041-1052, 2010. Go to original source...
    10. Houde, M., Belcaid, M., Ouellet, F., Danyluk, J., Monroy, A.F., Dryanova, A., Gulick, P., Bergeron, A., Laroche, A., Links, M.G., MacCarthy, L., Crosby, W.L., Sarhan, F.: Wheat EST resources for functional genomics of abiotic stress. - BMC Genomics 7: 149, 2006. Go to original source...
    11. Huang, J., Lu, X., Yan, H., Chen, S., Zhang, W., Huang, R.; Transcriptome characterization and sequencing-based identification of salt-responsive genes in Millettia pinnata, a semi-mangove plant. - DNA Res. 19:195-207, 2012. Go to original source...
    12. Hundertmark, M., Hincha, D.K.: LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. - BMC Genomics 9: 118, 2008. Go to original source...
    13. Ingram, J., Bartels, D.: The molecular basis of dehydration tolerance in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 377-403, 1996. Go to original source...
    14. Kim, E., Park, H.S., Jung, Y.J., Jeong, W.J., Park, H.S., Hwang, M.S., Park, E.J., Gong, Y.G., Choi, D.W.: Identification of the high-temperature response genes from Porphyra seriata (Rhodophyta) ESTs and enhancement of heat tolerance of Chlamydomonas (Chlorophyta) by expression of the Porphyra HTR2 gene. - J. Phycol. 47: 821-828, 2011. Go to original source...
    15. Kim, J.S., Kim, K.E., Oh, T.R., Park, C.M., Kang, H.; Functional characterization of DEAD-Box RNA helicases in Arabidopsis thaliana under abiotic stress conditions. - Plant Cell Physiol. 49: 1563-1571, 2008. Go to original source...
    16. Mortazavi, A., Williams, B.A., McCue, K., Schaeffer, L., Wold, B.: Mapping and quantifying mammalian transcriptomes by RNA-Seq. - Natur. Methods 5: 621-628, 2008. Go to original source...
    17. Nakashima, K., Yamaguchi-Shinozaki, K., Shinozaki, K.: The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold and heat. - Front. Plant Sci. 170: 1-7, 2014 Go to original source...
    18. Qin, D., Wu, H., Peng, H., Yao, Y., Ni, Z., Li, Z., Zhou, C., Sun, Q.: Heat stress-responsive trancriptome analysis in heat susceptible and tolerant wheat (Triticum aestivum L.) by using wheat genome array. - BMC Genomics 9: 432, 2008. Go to original source...
    19. Rodriguez, M.C.S., Edsga, D., Hussain, S.S., Alquezar1, D., Rasmussen, M., Gilbert, T., Nielsen, B.H., Bartels, D., Mundy, J.: Transcriptomes of the desiccation-tolerant resurrection plant Craterostigma plantagineum. - Plant J. 63: 212-228, 2010. Go to original source...
    20. Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi-Shinozaki, K., Carninci, P., Hayashizaki, Y., Shinozaki, K.; Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. - Plant Cell 13: 61-72, 2001. Go to original source...
    21. Shinozaki, K., Yamaguchi-Shinozaki, K.: Molecular response to dehydration and low temperature: difference and cross-talk between two stress signaling pathways. - Curr. Opin. Plant Biol. 2: 217-223, 2000. Go to original source...
    22. Vierling, E.: The roles of heat shock proteins in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579-620, 1991. Go to original source...
    23. Yamaguchi-Shinozaki, K., Shinozaki, K.: Organization of cisacting regulatory elements in osmotic- and cold-stress responsive promoters. - Trends Plant Sci. 10: 1360-1385, 2005. Go to original source...

    Return to the content