Abstract
T cell memory depends on factors that regulate expansion and death of these cells after antigenic stimulation. Mice deficient in perforin and interferon-gamma (IFN-gamma) exhibited increased expansion, altered immunodominance, and decreased death of antigen-specific CD8+ T cells after infection with an attenuated strain of Listeria monocytogenes, which was cleared from these mice. Expansion of CD8+ T cells was controlled by perforin, whereas IFN-gamma regulated immunodominance and the death phase. Thus, perforin and IFN-gamma regulate distinct elements of CD8+ T cell homeostasis independently of their role as antimicrobial effector molecules.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology
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Antigens, Bacterial / immunology
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Apoptosis
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / immunology*
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Homeostasis
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Immunodominant Epitopes / immunology*
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Immunologic Memory*
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Interferon-gamma / physiology*
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Listeria monocytogenes / immunology
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Listeriosis / immunology*
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Lymphocytic Choriomeningitis / immunology
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Membrane Glycoproteins / physiology*
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Perforin
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Pore Forming Cytotoxic Proteins
Substances
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Antigens, Bacterial
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Immunodominant Epitopes
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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Perforin
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Interferon-gamma