Dielectrophoretic tweezer for isolating and manipulating target cells
Dielectrophoretic tweezer for isolating and manipulating target cells
Date
2010-06-03
Authors
Menachery, Anoop
Graham, David M.
Messerli, Shanta M.
Pethig, Ronald
Smith, Peter J. S.
Graham, David M.
Messerli, Shanta M.
Pethig, Ronald
Smith, Peter J. S.
Linked Authors
Person
Person
Person
Person
Person
Alternative Title
Citable URI
As Published
Date Created
Location
DOI
Related Materials
Replaces
Replaced By
Keywords
Abstract
The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. We describe the design, theoretical modeling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells.. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI 193 human schwannoma cells, with visual identification of the target cells being aided by labeling the incorporated gene product with a green fluorescent protein.
Description
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Institution of Engineering and Technology for personal use, not for redistribution. The definitive version was published in IET Nanobiotechnology 5 (2011): 1-7, doi:10.1049/iet-nbt.2010.0010.