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Electrochemiluminescence immunosensor for ketamine detection based on polyamidoamine-coated carbon dot film

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Abstract

In this work, we reported the synthesis of water-soluble carbon dots (CDs) by a one-step hydrothermal synthetic route using polyamidoamine (PAMAM) as platform and passivant, where the formation of CDs capped with PAMAM and the surface passivation were realized simultaneously. As a result, the prepared PAMAM-CDs were uniform, monodispersed sphere particles with an approximate diameter of about 2 nm, and the fluorescence and electrochemiluminescence (ECL) response of the nanocomposite film were enhanced. By combining the high sensitivity of ECL and the selectivity of immunoreactions, an ECL immunosensor for facile, rapid, and ultrasensitive detecting of ketamine was constructed, and the further application of the immunoassay was investigated. Under optimal experimental conditions, the obtained immunosensor showed a wide linear detection range from 0.2 to 200 ng mL−1 with a detection limit at 0.067 ng mL−1 in 0.1 M phosphate buffer solutions (pH 7.4). The immunosensor was successfully applied to the determination of ketamine in blood plasma. This kind of assay is expected to pave new avenues in label-free drug assays.

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Acknowledgments

We gratefully acknowledge the financial support extended by the National Natural Science Foundation of China (No. 21475092), Natural Science Foundation of Jiangsu Province (BK2011273), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201207), and the Open Research Project of the Battery Technology Innovation Center for Public Services of Jiangsu Province (HDP201206).

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, People’s Republic of China

    Qianhua Li, Wenjie Tang, Yan Wang, Junwei Di & Ying Wu

  2. The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Suzhou, 215123, People’s Republic of China

    Qianhua Li, Wenjie Tang, Yan Wang, Junwei Di & Ying Wu

  3. Department of Anesthesiology of First Affiliated Hospital of Soochow University, Suzhou, 215006, People’s Republic of China

    Jianping Yang

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  1. Qianhua Li
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  2. Wenjie Tang
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  3. Yan Wang
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  4. Junwei Di
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  5. Jianping Yang
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  6. Ying Wu
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Correspondence to Ying Wu.

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Li, Q., Tang, W., Wang, Y. et al. Electrochemiluminescence immunosensor for ketamine detection based on polyamidoamine-coated carbon dot film. J Solid State Electrochem 19, 2973–2980 (2015). https://doi.org/10.1007/s10008-015-2913-9

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  • DOI: https://doi.org/10.1007/s10008-015-2913-9

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