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Simultaneous Determination of the Active Ingredients in Abelmoschus manihot (L.) Medicus by CZE

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Abstract

A capillary zone electrophoretic method has been developed for the quantitative analysis of four active compounds, quercetin-3-O-robinobioside (LXY3), hyperin (LXY4), isoquercetin (LXY5) and myricetin (LXY7) in A. manihot (L.) Medicus with UV detection at 254 nm. The capillary temperature was kept constant at 25 °C. The effects of buffer composition, pH, and concentration and running voltage on migration were studied systematically. Optimum separation was achieved with 20 mmol L−1 Borax–NaOH buffer (pH 9.50) at 25 °C and 25 kV. Regression equations revealed good linear relationship between the peak area of each compound and its concentration. All the correlation coefficients were higher than 0.9990. The relative standard deviations of migration time and the peak area were <0.53% and 5.34% (inter-day), and <0.56% and 3.81% (intra-day). The contents of the four compounds in A. manihot (L.) Medicus were successfully determined with satisfactory repeatability and recovery.

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Authors and Affiliations

  1. Department of Pharmacy, Peking University People’s Hospital, Beijing, 100044, China

    Yi Liu & Yuzhen Li

  2. Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China

    Wujie Li & Xiaomei Ling

  3. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China

    Xianyin Lai, Qingying Zhang & Yuying Zhao

Authors
  1. Yi Liu
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  2. Wujie Li
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  3. Xiaomei Ling
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  4. Xianyin Lai
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  5. Yuzhen Li
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  6. Qingying Zhang
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  7. Yuying Zhao
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Corresponding authors

Correspondence to Xiaomei Ling or Yuying Zhao.

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Liu, Y., Li, W., Ling, X. et al. Simultaneous Determination of the Active Ingredients in Abelmoschus manihot (L.) Medicus by CZE. Chroma 67, 819–823 (2008). https://doi.org/10.1365/s10337-008-0564-z

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  • DOI: https://doi.org/10.1365/s10337-008-0564-z

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