Summary
It has been found that, by addition of a certain amount of glycerol, it is possible to store living animal tissues in the frozen state over very long periods of time. The cooling velocity, the temperature of storage and the rate of thawing are important factors. Under the optimal conditions, experiments done on the heart of the chick embryo have shown that complete recovery of the normal physiological activities can be achieved after freezing in liquid nitrogen.
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References
J. Rostand, Rev. Gén. Froid35, 1187 (1958).
R. Andjus, C. R. Acad. Sci.232, 1591 (1951).
I. Giaja, Discours d'ouverture Symposium sur l'Hypothermie, XVe Congrès International de Médecine et Pharmacic Militaires (Belgrade 1957).
R. Andjus, Rev. Gén. Froid.36, 643 (1959).
V. Negovski etV. I. Sobolef, Annexe 1958–3, Suppl. au Bul. Inst. Inter. Froid, 75 (1958).
V. A. Negovski,Pathophysiologie und Therapie der Agonic und des klinischen Todes (Akademie Verlag Ed., Berlin 1959).
A. U. Smith, Proc. Roy. Soc. London, B.145, 391 (1956).
J. E. Lovelock etA. U. Smith, Proc. Roy. Soc. London B.145, 427 (1956).
L. R. Rey,Conservation de la vie par le froid (Herman Ed., Paris 1959).
J. E. Lovelock, Ciba Foundation Symposium (J. et A. Churchill, Ed., Londres 1954), p. 131.
C. Polge etJ. E. Lovelock, Vet. Record.64, 396 (1952).
H. Sloviter, cité parSmith 12.
A. U. Smith, dansBiological Applications of Freezing and Drying (R. J. C. Harris, Ed., Acad. Press., N. Y. 1954), p. 1.
H. T. Meryman, Proc. Roy. Soc. B.147, 452 (1957).
L. R. Rey, Bull. Inst. Inter. Froid34, 993 (1959).
B. J. Luyet, dansFreezing and Drying (R. J. C. Harris, Ed., the Institute of Biology, Londres 1951), p. 77.
H. Molisch, A. U. Smith, etA. S. Parkes, Flora97, 121 (1907).
L. Matruchot etM. Molliard, C. R. Acad. Sci.132, 495 (1901).
B. J. Luyet etP. M. Gehenio,Biodynamica (Ed. Normandy, Miss. 1940).
B. J. Luyet etF. Gonzalez, Biodynamica7, 61 (1951).
J. Rostand, C. R. Acad. Sci.234, 2310 (1946).
C. Polge, A. U. Smith etA. S. Parkes, Nature164, 666 (1949).
L. R. Rey, Proc. Roy. Soc. B.147, 460 (1957).
L. R. Rey, J. Embryol. exp. Morph.6, 171 (1958).
L. R. Rey etD. Simatos, C. R. Acad. Sci., Rio-de-Janciro, sous presse (1959).
L. R. Rey, Rev. Quest. Sci. Fr.18, 541 (1957).
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Rey, L.R. La conservation de la vie par le froid. Experientia 15, 449–455 (1959). https://doi.org/10.1007/BF02158248
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DOI: https://doi.org/10.1007/BF02158248