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La conservation de la vie par le froid

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Summary

It has been found that, by addition of a certain amount of glycerol, it is possible to store living animal tissues in the frozen state over very long periods of time. The cooling velocity, the temperature of storage and the rate of thawing are important factors. Under the optimal conditions, experiments done on the heart of the chick embryo have shown that complete recovery of the normal physiological activities can be achieved after freezing in liquid nitrogen.

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Rey, L.R. La conservation de la vie par le froid. Experientia 15, 449–455 (1959). https://doi.org/10.1007/BF02158248

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