We report the solution structure of the 37,500 Mr carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic E. coli, which in contrast to the crystal structure is a symmetric homopentamer in solution.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
ADP-ribosylating toxins (Aktories, K., ed) Curr. Topics in Microbiol. Immunol. 175, 1–143 (1992).
Lindberg, A.A. et al. J. Biol. Chem. 262, 1779–1785 (1987).
Waddell, T., Head, S., Petric, M., Cohen, A. & Lingwood, C. Biochem. Biophys. Res. Comm. 152, 674–679 (1988).
Samuel, J.E. et al. Infect. Immun. 58, 611–618 (1990).
Armstrong et. al. J. Infect. Dis. 171, 1042–1045 (1995).
Stein, P.E., Boodhoo, A., Tyrrell, G.J., Brunton, J.L. & Read, R.J. Nature 355, 748–750 (1992).
Ikura, M., Kay, L.E. & Bax, A. Biochemistry 29, 4659–4667 (1990).
Brünger, A.T. X-PLOR (3.1), Manual, Yale University, New Haven (1993).
Fraser, M.E., Chernaia, M.M., Kozlov, Y.V. & James, M.N.G. Nature Struct. Biol. 1, 59–64 (1994).
Kay, L.E., Torchia, D.A. & Bax, A. Biochemistry 28, 8972–8979 (1989).
Saleh, M.T. & Gariépy, J. J. Biochemistry 32, 918–922 (1993).
Hirst, T.R., Hilary, J.B., Ruddock, L.W. & Yu, J. Biochem. Soc. Trans. 23, 985–991 (1995).
Bhakuni, V., Xie, D. & Freire, E. Biochemistry 30, 5055–5060 (1991).
De Wolf, M., Van Dessel, G., Lagrou, A., Hilderson, H.J. & Dierick, W. Biochim. Biophys. Acta 832, 165–174 (1985).
Sandvig, K. et al. Nature 358, 510–512 (1992).
Ruddock, L.W. et al. Biochemistry, in the press.
Nyholm, P-G. et al. Chem. Biol. 3, 263–275 (1996).
Clark et al. Mol. Microbiol. 19, 891–899 (1996)
Perkins, S.J. in Biological Magnetic Resonance Vol. 4 (Berliner, L. & Reuben, J., Eds.) 193–336 (Plenum, New York, 1982).
Clore, G.M. & Gronenborn, A.M. J. Magn. Reson. 48, 402–417 (1982).
Ni, F. Progr. NMR Spectr. 26, 517–606 (1994).
London, R.E., Perlman, M.E. & Davis, D.G. J. Magn. Reson. 97, 79–98 (1992).
Calderwood, S.B. et al. A. Infect. Immun. 58, 2977–2982 (1990).
Acheson, D.W.K. et al. Infect. Immun. 61, 1098–1104 (1993).
Donohue-Rolfe, A. & Keusch, G.T. Infect. Immun. 39, 270–274 (1982).
Nilges, M. Proteins Struct., Funct. Genet. 17, 297–309 (1993).
Lasowski, R.A., MacArthur, M.W., Moss, D.S. & Thornton, J.M. Journal App. Crystallogr. 26, 283–291 (1993).
Frishman, D. & Argos, P. Proteins Struct., Funct., Genet. 23, 579 (1995).
Merritt, E.A. & Murphy, M.E.P. Acta Crystallogr. D50, 869–373 (1994).
Kraulis, P. Scientist 7, 16 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Richardson, J., Evans, P., Homans, S. et al. Solution structure of the carbohydrate-binding B-subunit homopentamer of verotoxin VT-1 from E. coli. Nat Struct Mol Biol 4, 190–193 (1997). https://doi.org/10.1038/nsb0397-190
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nsb0397-190