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Monitoring the preparation of NAD analogs by reversed-phase high-performance liquid chromatography

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Summary

Pig brain NAD glycohydrolase immobilized on Affi-Gel 10 or nylon 6 was used for the conversion of NAD into 3-acetylpyridine adenine dinucleotide (APAD) or 3-aminopyridine adenine dinucleotide (AAD).

A reversed-phase chromatographic system consisting of a C18 Resolve column and phosphate buffer (pH 6.2)-methanol as the mobile phase was used to monitor the production of APAD and AAD.

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Authors and Affiliations

  1. Dipartimento di Scienze e Tecnologie Biomediche-Sez. Chimica Organica, via Celoria, 2-20133, Milano, (Italy)

    P. G. Pietta, P. L. Mauri, M. Pace & D. Agnellini

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  1. P. G. Pietta
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  2. P. L. Mauri
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  3. M. Pace
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  4. D. Agnellini
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Pietta, P.G., Mauri, P.L., Pace, M. et al. Monitoring the preparation of NAD analogs by reversed-phase high-performance liquid chromatography. Chromatographia 25, 543–544 (1988). https://doi.org/10.1007/BF02324831

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  • DOI: https://doi.org/10.1007/BF02324831

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