Summary
A method is described for the determination of nitrated polynuclear aromatic hydrocarbons (nitro-PAHs), in particular 1-nitropyrene, in diesel particulate extracts. The method employs a multidimensional HPLC (column switching) technique with final on-line peak identification by UV-VIS spectral comparison with standards. To achieve exceptional chromatographic selectivity for nitro-PAHs, a new pyrene butyric acid amide phase has been prepared which is capable of forming donor-acceptor complexes with them. With this technique it is possible to confirm the presence of 1-nitropyrene in the range 3–100 ng/mg on filter-collected diesel soot. Its utility was demonstrated with diesel exhaust extracts spiked with varying levels of 1-nitropyrene and proved to be highly selective.
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Code of Federal Regulations 40, Part 86, U.S. Environmental Protection Agency.
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Dedicated to Prof. J. F. K. Huber at the occasion of his 60th birthday.
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Lindner, W., Posch, W., Wolfbeis, O.S. et al. Analysis of nitro-PAHs in diesel exhaust particulate extracts with multicolumn HPLC. Chromatographia 20, 213–218 (1985). https://doi.org/10.1007/BF02259689
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DOI: https://doi.org/10.1007/BF02259689