Summary
Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.
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Springer, M.S., Davidson, E.H. & Britten, R.J. Calculation of sequence divergence from the thermal stability of DNA heteroduplexes. J Mol Evol 34, 379–382 (1992). https://doi.org/10.1007/BF00162994
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DOI: https://doi.org/10.1007/BF00162994