Abstract
A recently-developed method for histological decalcification using aqueous solutions of basic chromium (III) sulphate has been applied to thin sections of adult human dentine. Subsequent studies in the electron microscope show a very good retention of morphological detail in the extracellular regions.
Special attention was given to the appearance of the peritubular matrix within which well-differentiated layers were recognised. There was substantial agreement between electron microscopy and recent studies of the same matric under the light microscope, allowing a novel hypothesis for the formation of the peritubular dentine.
Résumé
Une méthode nouvelle de décalcification histologique utilisant des solutions aqueuses de sulfate de chrome basique (III) est appliquée à des coupes fines de dentine humaine adulte. L'observation en microscopie électronique montre une bonne conservation structurale dans les régions extra-cellulaires. L'aspect de la matrice péricanaliculaire est particulièrement étudié. On y reconnait plusieurs couches bien individualisées. Une bonne concordance est notée entre les résultats obtenus par microscopie optique et électronique, permettant d'établir une hypothèse nouvelle sur la formation de la dentine péricanaliculaire.
Zusammenfassung
Eine kürzlich entwickelte Methode zur histologischen Entkalkung mittels wäßriger Lösungen von basischem Chrom III-Sulfat wird an dünnen Schliffen von menschlichem Dentin Erwachsener angewandt. Darauffolgende Untersuchungen am Elektronenmikroskop zeigten eine sehr gute Wiedergabe der morphologischen Einzelheiten in den Extrazellulär-Regionen.
Besonders beachtet wurde das Aussehen der peritubulären Matrix, innerhalb welcher gut differenzierte Schichten erkannt werden konnten. Eine wesentliche Übereinstimmung bestand zwischen Elektronenmikroskopie und kürzlich durchgeführten Untersuchungen an derselben Matric unter dem Mikroskop. Diese Methode ermöglicht es, eine neue Hypothese über die Bildung des peritubulären Dentins aufzustellen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allred, H.: The differential staining of peritubular and intertubular dentine matrices in human dentine. Arch. oral Biol.13, 1–12 (1968a).
—: The staining of lipids in human dentine matrix. Arch. oral Biol.13, 433–444 (1968b).
Bernard, G. W.: An electron microscopic study of initial intramembraneous osteogenesis in the mouse. Diss. Los Angeles: School of Dentistry, University of California 1968.
Bernard, G. W., Pease, D.: The bone nodule. JADR 45th General meeting, p. 72 (1967).
Bradford, E. W.: Microanatomy and histochemistry of dentine. In: Structural and chemical organization of teeth (A. E. W. Miles, ed.), vol. II, p. 3–34. New York: Academic Press 1967.
Eda, S., Takuma, S.: Microstructure of the peritubular matrix in horse dentin. Bull. Tokyo dent. Coll.6, 1–14 (1965).
Eastoe, J. E.: II. The matrix proteins in dentine and enamel from developing human deciduous teeth. Arch. oral Biol.8, 633–652 (1963).
—: Recent studies on the organic matrices of bone and teeth. In: First European Symposium on Bones and Teeth (H. J. J. Blackwood, ed.), p. 269. Oxford: Pergamon Press 1964.
—: Chemical aspects of the matrix concept in calcified tissue organisation. Calc. Tiss. Res.2, 1–19 (1968).
Frank, R. M.: Étude au microscope électronique de l'émail humain adult. Actualités odontostomat.45, 13–35 (1959).
—: Ultrastructure of human dentine. In: Calcified tissues 1965. (H. Fleish, H. J. J. Blackwood and M. Owen, eds.), p. 259–272. Berlin-Heidelberg-New York: Springer 1966.
Jansen, H. T.: An improved method for the preparation of “serial” sections of undecalcified dental tissues. J. dent. Res.29, 401–406 (1950).
Johansen, E.: Ultrastructure of dentine. In: Structural and chemical organization of tecth (A. E. W. Miles, ed.), vol. II, p. 35–74. New York: Academic Press 1967.
Longhi, L., Sasso, W. S.: Detesao histoquimica de mucopolissacarideous na zona pericanalicular (área translúcida de Bradford) da dentina humana. Rev. Fac. Odont. S. Paulo3, 11–18 (1965).
Sundström, B.: Histological decalcification using aqueous solutions of basic chromium (III) sulphate. Odont. Revy19, Suppl. 13 (1968).
Symons, N. B. B.: A histochemical study of the intertubular and peritubular matrices in normal human dentin. Arch. oral Biol.5, 241–250 (1961).
—: The microanatomy and histochemistry of dentinogenesis. In: Structural and chemical organization of teeth (A. E. W. Miles, ed.), vol. I, p. 285–324. New York: Academic Press 1967.
—: The formation of primary and secondary dentine. In: Dentine and pulp (N. B. B. Symons, ed.), p. 67–76. Dundee: D. C. Thompson & Co., Ltd. 1968.
Takuma, S.: Electron microscopy of the structure around the dentinal tubule. J. dent. Res.39, 973–981 (1960).
—: Ultrastructure of dentinogenesis: In. Structural and chemical organization of teeth (A. E. W. Miles, ed.), vol. I, p. 325–370. New York: Academic Press 1967.
—: Eda, S.: Structure and development of the peritubular matrix in dentin. J. dent. Res.45, 683–692 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sundström, B., Takuma, S. & Nagai, N. Ultrastructural aspects of human dentine decalcified with chromium sulphate. Calc. Tis Res. 4, 305–313 (1969). https://doi.org/10.1007/BF02279133
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02279133