Abstract
The ruffled border of osteoclasts in the fracture callus of rat consists of complex infoldings of the plasma membrane forming narrow channels. Absorption of an exogenous, electron-dense compound, thorium dioxide, has been shown to take place via these channels, apparently through “membrane flow”. The contents of the channels are transferred to lysosomes (“specific granules”) located subjacent to the ruffled border. In “transitional regions” adjacent to the ruffled border, uptake of thorium dioxide particles appeared to occur through “membrane vesiculation” (conventional endocytosis).
Résumé
La bordure en brosse des ostéoclastes de cals de fractures de rats présente des plissements complexes de la membrane cytoplasmique formant des canaux étroits. L'absorption d'un produit exogène opaque aux électrons (des macromolécules de dioxyde de thorium) s'effectue par l'intermédiaire de ces canaux, par un «courant» membranaire. Les contenus des canaux sont transférés à des lysosomes («granules spécifiques»), situés sous la bordure en brosse. Dans des «régions de transition», adjacentes à cette dernière, l'absorption de dioxyde de thorium se fait par «vésiculation membranaire» (endocytose classique).
Zusammenfassung
Der gekrauste Rand der Osteoklasten im Frakturcallus von Ratten besteht aus komplexen Einstülpungen der Plasmamembran, die enge Kanälchen bildet. Die Absorption einer exogenen, elektronisch dichten Verbindung, Thoriumdioxyd, erfolgt durch diese Kanäle, offenbar durch einen „Membranfluß”. Der Inhalt der Kanäle wird zu den Lysosomen („spezifische Granula”) geführt, welche unter dem gekrausten Rand liegen. In „Übergangsgebieten”, welche sich neben dem gekrausten Rand befinden, scheint die Aufnahme der Thoriumdioxydpartikel durch „Bläschenbildung in der Membran” (konventionelle Endocytose) stattzufinden.
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Göthlin, G., Ericsson, J.L.E. Observations on the mode of uptake of thorium dioxide particles by osteoclasts in fracture callus. Calc. Tis Res. 10, 216–222 (1972). https://doi.org/10.1007/BF02012551
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DOI: https://doi.org/10.1007/BF02012551