Summary
Permeation parameters of isolated cuticular membranes of Citrus aurantium L. for gaseous monoterpenes were determined by an isostatic system. For α-pinene and d-limonene permeability coefficients range from 4.3 × 10−11 m−2 s−1 to 7.3 × 10−11 m−2 s−1. These values can be compared to that measured for benzene gas at the cuticle of Citrus. The permeability coefficients of the two monoterpenes did not differ significantly, in contrast to their diffusioin coefficients. The diffusion coefficient values are 3.7 × 10−15 m−2 s−1 for limonene and 15.5 × 10−15 m−2 s−1 for α-pinene. The reason for this difference is still unclear. A dependence of the permeation parameters on the direction of the monoterpene transport could not be observed. Moreover, there are some indications that, in spite of its heterogeneous character, the cuticular membrane of Citrus is homogeneous in respect to the transport of small gaseous molecules. An exposure to environmentally relevant ozone concentrations for 6 months did not change the permeation characteristics of the membrane. Due to the high variability of the samples only a tendency towards higher permeability coefficients of cuticles treated with 80 ppb ozone was observed. This may be attributed to a reduced tension of the membrane caused by chain fractions.
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Atkinson R, Aschmann SM, Arey J (1990) Rate constants for the gas-phase reactions of OH and NO3 radicals and O3 with sabinene and camphene at 296±2K. Atmos Environ 24A: 2647–2654
Becker KH, Brockmann KJ, Bechara J (1990) Production of hydrogen peroxide in forest air by reaction of ozone with terpenes. Nature 346: 256–258
Becker M (1987) Permeabilität der pflanzlichen Kutikula. Bestimmung und Analyse der Transportparameter für lipophile organische Ver bindungen. Ph. D. thesis, Technische Universität, München
Becker M, Kerstiens G, Schönherr J (1986) Water permeability of plant cuticles: permeance, diffusion and partition coefficients. Trees 1: 54–60
Bischoff M (1984) Gaschromatographische und gravimetrische Untersuchung des Diffusions- und Permeationsverhaltens von Gasen und Dämpfen in Kunststoffen. Ph. D. thesis, Technische Hochschule, Stuttgart
Corchnoy SB, Atkinson R (1990) Kinetics of the gas-phase reactions of OH and NO3 radicals with 2-carene, 1,8-cineole, p-cymene, and terpinolene. Environ Sci Technol 24: 1497–1502
DeLassus PT (1985) Transport of unusual molecules in polymer films. Proceedings of the polymers, laminations and coatings conference. TAPPI 2: 445–450
Felder RM, Huvard GS (1980) Permeation, diffusion, and sorption of gases and vapors. In: Marton L, Marton C (eds) Methods of experimental physics. Polymers, Physical properties, vol. 16 C. Academic Press, New York, pp 315–377
Felder RM, Spence RD, Ferrell JK (1975) A method for the dynamic measurement of diffusivities of gases in polymers. J Appl Polym Sci 19: 3193–3200
Gäb S, Hellpointner E, Turner WV, Korte F (1985) Hydroxymethyl hydroperoxide and bis(hydroxymethyl) peroxide from gas-phase ozonolysis of naturally occurring alkenes. Nature 316: 535–536
Geyer U, Schönherr J (1990) The effect of the environment on the permeability and composition of Citrus leaf cuticle. I. Water permeability of isolated cuticular membranes. Planta 180: 147–153
Graedel TE (1979) Terpenoids in the atmosphere. Rev Geophys Space Phys 15: 937–947
Hanover J (1972) Factors affecting the release of volatile chemicals by forest trees. Mitt Forstl Bundes-Versuchsanst Wien 97: 625–644
Hernandez RJ, Giacin JR, Baner AL (1986) The evaluation of the aroma barrier properties of polymer films. J Plastic Film Sheeting 2: 187–211
Hewitt CN, Kok GL (1991) Formation and occurrence of organic hydroperoxides in the troposphere: laboratory and field observations. J Atmos Chem 12: 181–194
Jeffree CE, Johnson RPC, Jarvis PG (1971) Epicuticular wax in the stomatal antechamber of Sitka spruce and its effects on the diffusion of water vapour and carbon dioxide. Planta 98: 1–10
Kerler F, Schönherr J (1988) Permeation of lipophilic chemicals across plant cuticles: prediction from partition coefficients and molar volumes. Arch Environ Contam Toxicol 17: 7–12
Kerstiens G, Lendzian KJ (1989) Interactions between ozone and plant cuticles. I. Ozone deposition and permeability. New Phytol 112: 13–19
Lamb B, Guenther A, David G, Westberg H (1987) A national inventory of biogenic hydrocarbon emissions. Atmos Environ 21: 1695–1705
Lendzian KJ (1982) Gas permeability of plant cuticles. Oxygen permeability. Planta 155: 310–315
Lendzian KJ (1984) Permeability of plant cuticles to gaseous air pollutants. In: Koziol MJ, Whatley FR (eds) Gaseous air pollutants and plant metabolism. Butterworths, London, pp 77–81
Lendzian KJ, Kerstiens G (1988) Interactions between plant cuticles and gaseous air pollutants. Aspects Appl Biol 17: 97–104
Lendzian KJ, Kerstiens G (1991) Sorption and transport of gases and vapors in plant cuticles. Rev Environ Contam Toxicol 121: 65–128
Michelozzi M, Squillace AE, White TL (1990) Monoterpene composition and fusiform rust resistance in slash pine. For Sci 36: 470–475
Mohney SM, Hernandez RJ, Giacin JR, Harte BR, Miltz J (1988) Permeability and solubility of d-limonene vapor in cereal package liners. J Food Sci 53
Pasternak RA, Schimscheimer JF, Heller J (1970) A dynamic approach to diffusion and permeation measurements. J Polym Sci 8: 467–479
Payer HD, Bosch Chr, Blank LW, Eisenmann T, Runkel KH (1986) Beschreibung der Expositionskammern und der Versuchsbedingungen bei der Belastung von Pflanzen mit Luftschadstoffen und Klimastreß. Forstwiss Centralbl 105: 207–218
Raffa KF, Berryman AA, Simasko J, Teal W, Wong BL (1985) Effects of grand fir monoterpenes on the fir engraver. Scolytus ventralis (Coleoptera: Scolytidae) and its symbiotic fungus. Environ Entomol 14: 552–556
Reid RC, Prausnitz JM, Sherwood TK (1977) The properties of gases and liquids. McGraw-Hill, New York
Schmid C (1991) Sorptions- und Permeationseigenschaften der pflanzlichen Cuticula für Monoterpene. Ph. D. thesis, Technische Universität, München
Schmid C, Steinbrecher R, Ziegler H (1991) Partition coefficients of plant cuticles for monoterpenes. Trees (in press)
Schönherr J (1976) Water permeability of isolated cuticular membranes: the effect of cuticular waxes on diffusion of water. Planta 131: 159–164
Schönherr J (1982) Resistance of plant surfaces to water loss: transport properties of cutin, suberin and associated lipids. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Encyclopedia of plant physiology. Springer, Berlin Heidelberg New York, pp 153–179
Schönherr J, Riederer M (1986) Plant cuticles sorb lipophilic compounds during enzymatic isolation. Plant Cell Environ 9: 459–466
Schönherr J, Riederer M (1988) Desorption of chemicals from plant cuticles: evidence for asymmetry. Arch Environ Contam Toxicol 17: 13–19
Schönherr J, Riederer M (1989) Foliar penetration and accumulation of organic chemicals in plant cuticles. Rev Environ Contam Toxicol 18: 1–70
Schreiber L, Schönherr J (1990) Phase transition and thermal expansion coefficients of plant cuticles. The effects of temperature on structure and function. Planta 182: 186–193
Steinbrecher R (1989) Gehalt und Emission von Monoterpenen in oberirdischen Organen von Picea abies (L.) Karst. Ph. D. thesis, Technische Universität, München
Tyree MT, Scherbatskoy TD, Tabor CA (1990) Leaf cuticles behave as asymmetric membranes. Evidence from the measurement of diffusion potentials. Plant Physiol 92: 103–109
Ziegel KD, Frensdorff HK, Blair DE (1969) Measurement of hydrogen isotope transport in poly(vinyl fluoride) films by the permeation-rate method. J Polym Sci 7: 809–819
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This paper is dedicated to Prof. Dr. Otto Härtl, Graz, on the occasion of his 80th birthday.
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Schmid, C., Ziegler, H. Permeation characteristics of isolated cuticles of Citrus aurantium L. for monoterpenes. Trees 6, 172–177 (1992). https://doi.org/10.1007/BF00202433
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DOI: https://doi.org/10.1007/BF00202433