Summary
A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.
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Cherif, D., Bernard, O. & Berger, R. Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes. Hum Genet 81, 358–362 (1989). https://doi.org/10.1007/BF00283691
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DOI: https://doi.org/10.1007/BF00283691