Summary
The differentiation of muscles in primary cultures of cells fromDrosophila melanogaster embryos was investigated. In early cultures, and in the absence of exogenous ecdysone, two main classes of muscle were found. Comparison, by light and electron microscopy, of one of these classes (the “myotube” class) with muscles from third instar larvae shows that this class corresponds to the muscles of the body wall of the larva. When α- or β-ecdysone is added to the cultures, these undergo a number of metamorphic changes. Most of the larval muscles disappear, and two new types of muscle form. Ultrastructural and light microscopic examination of these two types indicates that they correspond to the two classes of skeletal muscle (fibrillar and tubular) found in adult flies.
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Auber, J.: La myofibrillogenèse du muscle strié—I. Insectes. J. Microsc.8, 197–223 (1969)
Beinbrech, G.: Elektronenmikroskopische Untersuchungen über die Differenzierung von Insektenmuskeln während der Metamorphose. Z. Zellforsch.90, 463–494 (1968)
Crossley, A.C.: The fine structure and mechanism of breakdown of larval intersegmental muscles in the blowflyCalliphora erythrocephala. J. Insect Physiol.14, 1389–1407 (1968)
Deak, I.I.: Mutations ofDrosophila melanogaster that affect muscles. J. Embryol. Exp. Morphol.40, 35–63 (1977)
Deak, I.I.: Thoracic duplications in the mutantwingless ofDrosophila and their effect on muscles and nerves. Dev. Biol., 1978 (accepted per publication)
Donady, J.J., Seecof, R.L.: Effect of the genelethal (1)myospheroid onDrosophila embryonic cells in vitro. In Vitro8, 7–12 (1972)
Dübendorfer, A.: Metamorphosis of imaginal disc tissue grown in vitro from dissociated embryos ofDrosophila. In: Invertebrate Tissue Culture. Applications in Medicine, Biology and Agriculture. New York: Acad. Press 1976
Dübendorfer, A., Shields, G., Sang, J.H.: Development and differentiation in vitro ofDrosophila imaginal disc cells from dissociated early embryos. J. Embryol. Exp. Morphol.33, 487–498 (1975)
Elder, H.Y.: In: Insect muscle (P.N.R. Usherwood, ed.), New York: Acad. Press 1975
Gerson, I., Seecof, R.L., Teplitz, R.L.: Ultrastructural differentiation during embryonicDrosophila myogenesis in vitro. In Vitro12, 615–622 (1976)
Goldstein, M.A.: An ultrastructural study of supercontraction in the body wall muscles ofDrosophila melanogaster larvae. Anat. Rec.169, 326 (1971)
Goldstein, M.A., Burdette, W.J.: Striated visceral muscle ofDrosophila melanogaster. J. Morphol.134, 315–334 (1971)
Graham-Smith, G.S.: The alimentary canal ofCalliphora erythrocephala L., with special reference to its musculature and to the proventriculus, rectal value and rectal papillae. Parasitology26, 176–248 (1934)
Hotta, Y., Benzer, S.: Mapping of behaviour inDrosophila mosaics. Nature (London)240, 527–535 (1972)
Miller, A.: In: Biology ofDrosophila (M. Demerec, ed.) New York: Hafner 1950
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. Appl. Phys.32, 1637 (1961)
Nüesch, H.: The role of the nervous system in insect morphogenesis and regeneration. A. Rev. Ent.13, 27–44 (1968)
Osborne, M.P.: Supercontraction in the muscles of the blowfly larva: an ultrastructural study. J. Insect Physiol.13, 1471–1482 (1967)
Pasquali-Ronchetti, I.: The ultrastructural organization of femoral muscles inMusca domestica (Diptera). Cell Tiss. Res.2, 339–354 (1970)
Robertson, C.W.: The metamorphosis ofDrosophila melanogaster, including an accurately timed account of the principal morphological changes. J. Morphol.59, 351–400 (1936)
Sahota, T.S., Beckel, W.E.: The influence of epidermis on the developing flight muscles inGalleria mellonella. Can. J. Zool.45, 421–434 (1967)
Seecof, R.L., Alléaume, N., Teplitz, R.L., Gerson, I.: Differentiation of neurons and myocytes in cell cultures made fromDrosophila gastrulae. Exp. Cell Res.69, 161–173 (1971)
Seecof, R.L., Gerson, I., Donady, J.J., Teplitz, R.L.:Drosophila myogenesis in vitro: the genesis of “small” myocytes and myotubes. Dev. Biol.35, 250–261 (1973)
Seecof, R.L., Teplitz, R.L., Gerson, I., Ikeda, K., Donady, J.J.: Differentiation of neuromuscular junctions in cultures of embryonicDrosophila cells. Proc. Natl. Acad. Sci. USA62, 566–570 (1972)
Seecof, R.L., Unanue, R.L.: Differentiation of embryonicDrosophila cells in vitro. Exp. Cell Res.50, 654–660 (1968)
Shafiq, S.A.: Electron microscopic studies on the indirect flight muscles ofDrosophila melanogaster. I. Structure of the myofibrils. J. Cell Biol.17, 351–362 (1963a)
Shafiq, S.A.: Electron microscopic studies on the indirect flight muscles ofDrosophila melanogaster. II. Differentiation of myofibrils. J. Cell Biol.17, 363–373 (1963b)
Shields, G., Dübendorfer, A., Sang, J.H.: Differentiation in vitro of larval cell types from early embryonic cells ofDrosophila melanogaster. J. Embryol. Exp. Morphol.33, 159–175 (1975)
Shields, G., Sang, J.H.: Characteristics of five cell types appearing during in vitro culture of embryonic material fromDrosophila melanogaster. J. Embryol. Exp. Morphol.23, 53–69 (1970)
Shields, G., Sang, J.H.: Improved medium for culture ofDrosophila embryonic cells. Dros. Inform. Serv.52, 161 (1977)
Tiegs, O.W.: The flight muscles of insects — their anatomy and histology; with some observations on the structure of striated muscle in general. Phil. Trans. Roy. Soc., London, Ser. B.238, 221–348 (1955)
Usherwood, P.N.R.: Insect muscle. New York: Acad. Press 1975
Yaffe, D.: Cellular aspects of muscle differentiation in vitro. Curr. Top. Dev. Biol.4, 37–77 (1969)
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Dübendorfer, A., Blumer, A. & Deàk, I.I. Differentiation in vitro of larval and adult muscles from embryonic cells ofDrosophila . Wilhelm Roux' Archiv 184, 233–249 (1978). https://doi.org/10.1007/BF00848256
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DOI: https://doi.org/10.1007/BF00848256