Summary
The evolution of the proflavine triplet state population with the extent of aggregation is examined, by ESR measurements, as a function of organic solvents and mineral salts, well known for their ability to influence the aggregation process.
The intensity of the triplet state population has also been investigated as a function ofpH. The insertion of aromatic molecules like DNA's nucleotides between dye molecules is shown to increase the intensity of the triplet state. It is put forward that the observed triplet state is characteristic of the singly protonated proflavine which seems to be the active species inin vivo experiments.
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Lion, Y., Saucin, M. & Van de Vorst, A. Triplet state population study in relation to the aggregation of proflavine in frozen solutions. Radiat Environ Biophys 12, 337–344 (1975). https://doi.org/10.1007/BF01323421
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DOI: https://doi.org/10.1007/BF01323421