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The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c

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Summary

The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differentially regulated by the product of CYP1. The HAP1 gene codes for a product which has previously been proved to be necessary for the expression of the heme dependent CYC1-UAS1 cis regulatory sequence. In this article, we show by complementation and recombination that CYP1 and HAP1 are the same gene, moreover we identify hap1-1 as an iso2-cytochrome c underproducer mutation of the CYP1 gene.

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Authors and Affiliations

  1. Centre de Génétique Moléculaire du C. N. R. S., Laboratoire pro pre associé a l'Université Pierre et Marie Curie, F-91190, Gif-sur-Yvette, France

    J. Verdière, F. Creusot & P. P. Slonimski

  2. Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    L. Guarente

Authors
  1. J. Verdière
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  2. F. Creusot
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  3. L. Guarente
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  4. P. P. Slonimski
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Verdière, J., Creusot, F., Guarente, L. et al. The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c. Curr Genet 10, 339–342 (1986). https://doi.org/10.1007/BF00418404

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  • DOI: https://doi.org/10.1007/BF00418404

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