Skip to main content
Log in

Origin of sclerotia-like cells in submerged Claviceps purpurea producing clavine alkaloids

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Ultrathin sectioning of submerged mycelium of Claviceps purpurea Tul. producing clavine alkaloids revealed yeast-like budding resulting in asexual sporesblastospores. These deciduous spores were born by extended hyphal cells and retained the same ultrastructure of cell organelles. Both the extended hyphae and the blastospores resembled the cells of ergot sclerotial tissue. A surface culture of C. purpurea Tul. producing no alkaloids was used as a reference.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ainsworth, G. C.: Dictionary of the fungi, 5th ed. Commonwealth Mycological Institute (1966)

  • Bauer, H., Sigarlakie, E.: Localization of alkaline phosphatase in Saccharomyces cerevisiae by means of ultrathin frozen sections. J. Ultrastruct. Res. 50, 208–215 (1975)

    Google Scholar 

  • Buckley, P. M., Sjaholm, V. E., Sommer, N. F.: Electron microscopy of Botrytis cinerea conidia. J. Bact. 91, 2037–2044 (1966)

    Google Scholar 

  • Corbett, K., Dickerson, A. G., Mantle, P. G.: Metabolic studies on Claviceps purpurea during parasitic development on rye. J. gen. Microbiol. 84, 39–58 (1974)

    Google Scholar 

  • Garrison, R. G., Boyd, K. S.: Dimorphism of Penicillium marneffei as observed by electron microscopy. Canad. J. Microbiol. 19, 1305–1309 (1973)

    Google Scholar 

  • Gay, J. L., Greenwood, A. D., Heath, I. B.: The formation and behaviour of vacuoles (vesicles) during oosphere development and zoospore germination in Saprolegnia. J. gen. Microbiol. 65, 233–241 (1971)

    Google Scholar 

  • Grove, S. N., Oujezdsky, K. B., Szanizslo, P. J.: Budding in the dimorphic fungus Phialophora dermatitis. J. Bact. 115, 323–329 (1973)

    Google Scholar 

  • Komersová, I., Ludvík, J., Majer, J.: The ultrastructure of spores of Claviceps purpurea (Fr.) Tul. Folia microbiol. (Praha) 14, 141–144 (1969)

    Google Scholar 

  • Mantle, P. G., Tonolo, A.: Relationship between the morphology of Claviceps purpurea and the production of alkaloids. Trans. Brit. mycol. Soc. 51, 499–505 (1968)

    Google Scholar 

  • Řeháček, Z., Kozová, J., Sajdl, P., Voříšek, J.: The physiology of conidial formation in submerged cultures of Claviceps purpurea (Fr.) Tul. producing alkaloids. Canad. J. Microbiol. 20, 1323–1329 (1974)

    Google Scholar 

  • Řeháček, Z., Sajdl, P., Kozová, J., Malik, K. A., Řičicová, A.: Correlation of certain alterations in metabolic activity with alkaloid production by submerged Claviceps. Appl. Microbiol. 22, 949–956 (1971)

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Salema, R., Brandão, I.: The use of Pipes buffer in the fixation of plant cells for electron microscopy. J. Submicr. Cytol. 5, 76–96 (1973)

    Google Scholar 

  • Spalla, C.: Genetic problems of production of ergot alkaloids in saprophytic and parasitic conditions. In: Genetics of industrial microorganisms (Z. Vaněk, Z. Hošťálek, J. Cudlín, eds.), pp. 393–403. Amsterdam: Elsevier 1973

    Google Scholar 

  • Voříšek, J., Ludvík, J., Řeháček, Z.: Morphogenesis and ultrastructure of Claviceps purpurea during submerged alkaloid formation. J. Bact. 120, 1401–1408 (1974)

    Google Scholar 

  • Voříšek, J., Pokorný, V.: Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi. Arch. Microbiol. 102, 293–298 (1975)

    Google Scholar 

  • Zaki, A. I., Eckert, J. W., Endo, R. M.: The ultrastructure of germinating conidia of Penicillium digitatum inhibited by secbutylamine. Pesticide Biochem. Physiol. 3, 7–13 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Voříšek, J., Řeháček, Z. Origin of sclerotia-like cells in submerged Claviceps purpurea producing clavine alkaloids. Arch. Microbiol. 107, 321–327 (1976). https://doi.org/10.1007/BF00425347

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00425347

Key words

Navigation