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Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa

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Summary

The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contains two gliadin loci, one (Gli-B1) coding for at least six ω- or γ-gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM. Amongst the chromosome 1A gliadins, three proteins are encoded at the Gli-A1 locus whereas polypeptides N14-N15-N16 are controlled by a remote locus which recombines with Gli-A1. Six other gliadins are controlled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cultivars sharing the Gli-B1 allele of Neepawa were found to differ in the presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a reducing agent during two-dimensional sodium dodecyl sulphate polyacrylamid-gel electrophoresis, suggesting the absence of intra-chain disulphide bonds in their structure.

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Authors and Affiliations

  1. Sezione di S. Angelo Lodigiano, Istituto Sperimentale Cerealicoltura, via Mulino 3, 20079, S. Angelo Lodigiano, Milano, Italy

    T. Dachkevitch, R. Redaelli, A. M. Biancardi & E. V. Metakovsky

  2. Sezione di Genetica Applicata, Istituto Sperimentale Cerealicoltura, via Cassia 176, 00100, Roma, Italy

    N. E. Pogna

Authors
  1. T. Dachkevitch
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  2. R. Redaelli
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  3. A. M. Biancardi
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  4. E. V. Metakovsky
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  5. N. E. Pogna
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Communicated by F. Salamini

Research supported by a grant from the Commission of the European Communities, ECLAIR programme, Contract AGRE 0052

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Dachkevitch, T., Redaelli, R., Biancardi, A.M. et al. Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa. Theoret. Appl. Genetics 86, 389–399 (1993). https://doi.org/10.1007/BF00222107

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  • DOI: https://doi.org/10.1007/BF00222107

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