Abstract
The effects of catecholamines on intracellular Ca2+concentrations ([Ca2+]i) in single acutely dissociated bovine adrenal medulla endothelial cells (BAMECs) were measured using the intracellular fluorescent probe Fluo-3 AM. 100 μm epinephrine or norepinephrine induced a biphasic [Ca2+]i rise with an initial peak followed by a delayed phase. 10 μm phenylephrine (α1-adrenergic agonist) caused a [Ca2+]i rise similar to that evoked by catecholamines. The increase in [Ca2+]i induced by 10 μm phenylephrine was reverted by 10 μm phenoxybenzamine (α-adrenergic antagonist). Neither isoproterenol (β-adrenergic agonist) nor clonidine (α2-adrenergic agonist) induced [Ca2+]i rise. The initial peak was insensitive to zero external Ca2+ and it was abolished after Ca2+ internal storages were emptied by 10 mM caffeine. The delayed phase was reduced to near zero by external Ca2+ removal. These results indicate that BAMECs possess α1-adrenergic receptors associated to both the release of caffeine-sensitive intracellular Ca2+ stores and the entry of extracellular Ca2+ We suggest that chromaffin cell secretion may activate BAMECs in vivo through an increase in [Ca2+]i which could induce the secretion of vasoactive factors allowing a rapid entry of hormones into the circulation. (Mol Cell Biochem 000: 000-000,1999)
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Vinet, R., Rojas, F., Luxoro, M. et al. Catecholamines-evoked cytosolic ca2+rise in endothelial cells from bovine adrenal medulla. Mol Cell Biochem 203, 53–58 (2000). https://doi.org/10.1023/A:1007089415685
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DOI: https://doi.org/10.1023/A:1007089415685