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Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

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Summary

Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploid S.tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

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  1. A. Carrasco

    Present address: APPACALE S.A., Valle de Mena, 13.09001, Burgos, Spain

Authors and Affiliations

  1. NEIKER-CIMA, Apdo 46, 01080, Vitoria, Spain

    A. Carrasco, J. I. Ruiz de Galarreta, A. Rico & E. Ritter

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  1. A. Carrasco
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Carrasco, A., Ruiz de Galarreta, J.I., Rico, A. et al. Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion. Potato Res 43, 31–42 (2000). https://doi.org/10.1007/BF02358511

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