Skip to main content
SpringerLink
Log in

Immunocytochemical localization of nerve growth factor in mouse salivary glands

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The submandibular glands of female mice and the sublingual and parotid glands of adult male and female mice have been examined by light microscopical immunocytochemistry for nerve growth factor (NGF). In female submandibular glands, staining for NGF was observed in granular convoluted tubule and striated duct cells. Sublingual glands of the mouse contained relatively few granular cells staining for NGF compared with submandibular glands. However, such granular cells appeared to be more numerous in male sublingual glands than in female glands. The remainder of the intralobular duct cells in both male and female sublingual glands exhibited apical subluminal staining for NGF as well as light basal plasmalemmal staining. Parotid glands in both male and female mice exhibited a similar pattern of staining for NGF in striated duct cells. However, the glands did not contain granular cells nor did they exhibit any pattern of staining which reflected a sexual dimorphism. Immunodot staining of salivary gland extracts confirmed the presence of immunoreactivity for NGF in all three of the major salivary glands.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • BARKA, T. (1980) Biologically active polypeptides in submandibular glands.J. Histochem. Cytochem. 28, 836–59.

    Google Scholar 

  • BERKMAN, M. D. & KRONMAN, J. H. (1970) A histochemical study of the effects of castration and testosterone administration on the major salivary glands of Swiss mice.Acta Anat. 76, 200–19.

    Google Scholar 

  • BHOOLA, K. D., DOREY, G. & JONES, C. W. (1973) The influence of androgens on enzymes (chymotrypsin-and trypsin-like proteases, renin, kallikrein, and amylase) and on cellular structure of the mouse submaxillary gland.J. Physiol. 235, 503–22.

    Google Scholar 

  • BOCCHINI, V. & ANGELETTI, P. U. (1969) The nerve growth factor: purification as a 30000-molecular weight protein.Proc. Natl. Acad. Sci. USA 64, 787–94.

    Google Scholar 

  • BRADFORD, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72, 248–54.

    Google Scholar 

  • CARAMIA, F. (1966) Ultrastructure of mouse submaxillary gland. I. Sexual differences; II. Effect of castration in the male.J. Ultrastruc. Res. 16, 505–23; 524–36.

    Google Scholar 

  • CHRÉTIEN, M. (1977) Action of testosterone on the differentiation and secretory activity of a target organ: the submaxillary gland of the mouse.Int. Rev. Cytol. 50, 333–96.

    Google Scholar 

  • CUTLER, L. S. (1980) The development and independent relationships between cytodifferentiation and morphogenesis in developing salivary gland secretory cells.Anat. Rec. 196, 341–7.

    Google Scholar 

  • GOLDSTEIN, M. N. & BURDMAN, J. A. (1965) Studies of the nerve growth factor in submandibular glands of female mice treated with testosterone.Anat. Rec. 151, 199–208.

    Google Scholar 

  • GRESIK, E. & BARKA, T. (1977) Immunocytochemical localization of epidermal growth factor in mouse submandibular glands.J. Histochem. Cytochem. 25, 1027–35.

    Google Scholar 

  • GRESIK, E. W. & BARKA, T. (1983) Epidermal growth factor, renin, and protease A in hormonally responsive duct cells of the mouse sublingual gland.Anat. Rec. 205, 169–75.

    Google Scholar 

  • GRESIK, E. W. & MACRAE, E. K. (1975) The postnatal development of the sexually dimorphic duct system and of amylase activity in the submandibular glands of mice.Cell Tissue Res. 157, 411–22.

    Google Scholar 

  • GRESIK, E., MICHELAKIS, A., BARKA, T. & ROSS, T. (1978) Immunocytochemical localization of renin in the submandibular gland of the mouse.J. Histochem. Cytochem. 26, 855–61.

    Google Scholar 

  • GRESIK, E. W, SCHENKEIN, I. & BARKA, T. (1981) Immunocytochemical investigations on the submandibular glands of developing and adult mice using a specific antiserum to protease. A.J. Histochem. Cytochem. 29, 1411–17.

    Google Scholar 

  • HAZEN-MARTIN, D. J. (1983) The effect of secretagogue stimulation on the granular tubule segment of the male mouse submandibular gland: an ultrastructural and immunocytochemical study.Anat. Rec. 205, 78–79A.

    Google Scholar 

  • HAZEN-MARTIN, D. J. & SIMSON, J. A. V. (1984) Immunocytochemical localization of nerve growth factor: effects of fixation.J. Histochem. Cytochem. 32, 30–6.

    Google Scholar 

  • HOFMANN, H.-D. & DRENCKHAHN, D. (1981) Distribution of nerve growth factor in the submandibular gland of the male and female mouse.Cell Tissue Res. 221, 77–83.

    Google Scholar 

  • JACOBY, F. & LEESON, C. R. (1959) The post-natal development of the rat submaxillary gland.J. Anat. 93, 201–16.

    Google Scholar 

  • JUNQUEIRA, C., FAJER, A., RABINOVITCH, M. & FRANK-ENTHAL, L. (1949) Biochemical and histochemical observations on the sexual dimorphism of mice submaxillary glands.J. Cell Comp. Physiol. 34, 129–58.

    Google Scholar 

  • KOERKER, R. M. (1967) The effects of hypophysectomy on the digestive glands of the mouse.Am. J. Anat. 121, 571–600.

    Google Scholar 

  • LACASSAGNE, A. (1940) Dimorphisme sexual de la glande sous maxillaire chez la souris.C. R. Soc. Biol., Paris 133, 180–81.

    Google Scholar 

  • LANGUNOFF, D., BENDITT, E. P. & WATTS, R. M. (1962) Histochemical study of esterases homospecific with trypsin.J. Histochem. Cytochem. 10, 672–3.

    Google Scholar 

  • LAWSON, K. A. (1972) The role of mesenchyme in the morphogenesis and functional differentiation of rat salivary epithelium.J. Embryol. Exp. Morph. 27, 497–513.

    Google Scholar 

  • LEVI-MONTALCINI, R. (1964) The nerve growth factor.Ann. N.Y. Acad. Sci. 118, 149–70.

    Google Scholar 

  • MASON, T. E., PHIFER, R. F., SPICER, S. S., SWALLOW, R. A. & DRESKIN, R. B. (1969) An immunoglobulinenzyme bridge method for localizing tissue antigens.J. Histochem. Cytochem. 17, 563–9.

    Google Scholar 

  • OGER, J., ARNASON, B. G. W., PANTAZIS, N., LEHRICH, J. & YOUNG, M. (1974) Synthesis of nerve growth factor by L and 3T3 cells in culture.Proc. Natl. Acad. Sci. USA 71, 1554–8.

    Google Scholar 

  • SCHWAB, M. E., STOCKEL, K. & THOENEN, H. (1976) Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy.Cell Tissue Res. 169, 289–99.

    Google Scholar 

  • SIMSON, J. A. V., HAZEN, D., SPICER, S. S., MURPHY, R. A. & YOUNG, M. (1978) Secretagogue-mediated discharge of nerve growth factor from granular tubules of male mouse submandibular glands: An immunocytochemical study.Anat. Rec. 192, 375–88.

    Google Scholar 

  • SIMSON, J. A. V., SPICER, S. S., CHAO, J., GRIMM, L. & MARGOLIUS, H. S. (1979) Kallikrein localization in rodent salivary glands and kidney with the immunoglobulin-enzyme bridge technique.J. Histochem. Cytochem. 27, 1567–76.

    Google Scholar 

  • SIMSON, J. A. V., FENTERS, R. & CHAO, J. (1983) Electron microscopic immunostaining of kallikrein in rat submandibular glands.J. Histochem. Cytochem. 31, 301–6.

    Google Scholar 

  • VREUGDENHIL, A. P., DELANGE, G. L., NIEUW AMERONGEN, A. V. & ROUKEMA, P. A. (1980) Morphological changes in the salivary glands upon stimulation by receptor-selective agonists. III. Sublingual glands of the mouse.J. Biol. Buccale 8, 87–98.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina, USA

    D. J. Hazen-Martin

  2. Department of Neurology, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina, USA

    G. Landreth

  3. Department of Anatomy, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina, USA

    J. A. V. Simson

Authors
  1. D. J. Hazen-Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Landreth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. A. V. Simson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hazen-Martin, D.J., Landreth, G. & Simson, J.A.V. Immunocytochemical localization of nerve growth factor in mouse salivary glands. Histochem J 19, 210–216 (1987). https://doi.org/10.1007/BF01680631

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01680631

Keywords

Search

Navigation