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Nuclear localization and signalling activity of phosphoinositidase Cβ in Swiss 3T3 cells

Nature volume 358, pages 242–245 (1992)Cite this article

Abstract

THE hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) is a widespread receptor-coupled signalling system at the plasma membrane of most eukaryotic cells. The existence of an entirely separate nuclear phosphoinositide signalling system is suggested from evidence that purified nuclei synthesize PtdInsP2and phosphatidylinositol 4-phosphate (PtdlnsP) in vitro1and that a transient decrease in the mass of these lipids occurs when Swiss 3T3 cells are cultured in the presence of insulin-like growth factor-1 (IGF-1)2–4. These IGF-1-dependent changes in inositol lipids coincide with an increase in nuclear diacylglycerol4 and precede translocation to the nucleus and activation of protein kinase C (refs 5, 6). Circumstantial evidence that links these changes with mitosis comes from the isolation of a 3T3 clone that expresses the type-1 IGF receptor and binds IGF-1 peptide but does not respond mitogenically or show transient mass changes in nuclear inositol lipids7. A key question is how IGF-1 initiates the rapid breakdown of PtdlnsP and PtdlnsP2, in the nucleus. Here we present evidence that nuclei of 3T3 cells contain the β-isozyme of phosphoinositidase C, whereas the γ-isozyme is confined to the cytoplasm and that IGF-1 treatment stimulates exclusively the activity of nuclear phosphoinositidase C.

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References

  1. Cocco, L., Gilmour, R. S., Ognibene, A., Manzoli, F. A. & Irvine, R. F. Biochem. J. 248, 765–770 (1987).

    Article  CAS  Google Scholar 

  2. Manzoli, F. A. et al. Adv. Enz. Reg. 28, 25–34 (1989).

    Article  CAS  Google Scholar 

  3. Cocco, L. et al. Biochem. biophys. Res. Commun. 159, 720–725 (1989).

    Article  CAS  Google Scholar 

  4. Divecha, N., Banfic, H. & Irvine, R. F. EMBO J. 10, 3207–3214 (1991).

    Article  CAS  Google Scholar 

  5. Martelli, A. M. et al. Biochem. biophys. Res. Commun. 177, 480–487 (1991).

    Article  CAS  Google Scholar 

  6. Martelli, A. M., Gilmour, R. S., Falcieri, E., Manzoli, F. A. & Cocco, L. Expl Cell Res. 185, 191–202 (1989).

    Article  CAS  Google Scholar 

  7. Martelli, A. M. et al. FEBS Lett. 283, 243–246 (1991).

    Article  CAS  Google Scholar 

  8. Bennet, C. F. & Crooke, S. T. J. biol. Chem. 262, 13789–13797 (1987).

    Article  Google Scholar 

  9. Tucker, R. W. & Fay, F. S. Eur. J. Cell Biol. 51, 120–127 (1990).

    CAS  PubMed  Google Scholar 

  10. Suh, P. G., Ryu, S. H., Choi, W. C., Lee, K. Y. & Rhee, S. G. J. biol. Chem. 263, 14497–14504 (1988).

    Article  CAS  Google Scholar 

  11. Rhee, S. G., Suh, P. G., Ryu, S. H. & Lee, S. Y. Science 244, 546–550 (1989).

    Article  ADS  CAS  Google Scholar 

  12. Bansal, V. S. & Majerus, P. W. A. Rev. Cell Biol. 6, 41–67 (1990).

    Article  CAS  Google Scholar 

  13. Kim, U-H. et al. J. biol. Chem. 266, 1359–1362 (1991).

    Article  CAS  Google Scholar 

  14. McBride, K., Rhee, S. G. & Jaken, S. Proc. natn. Acad. Sci. U.S.A. 88, 7111–7115 (1991).

    Article  ADS  CAS  Google Scholar 

  15. Garland, R. C. & Cori, C. F. Biochemistry 11, 4712–4718 (1972).

    Article  CAS  Google Scholar 

  16. Thorne-Tjomsland, G., Clermont, Y. & Tang, X. Biol. Cell 71, 33–41 (1991).

    Article  CAS  Google Scholar 

  17. Smrcka, A. V., Hepler, J. R., Brown, K. O. & Sternweis, P. C. Science 251, 804–807 (1991).

    Article  ADS  CAS  Google Scholar 

  18. Taylor, S., Chae, H., Rhee, S. & Exton, J. Nature 350, 516–518 (1991).

    Article  ADS  CAS  Google Scholar 

  19. Wu, D., Lee, C. H., Rhee, S. G. & Simon, M. I. J. biol. Chem. 267, 1811–1817 (1992).

    Article  CAS  Google Scholar 

  20. Shenker, A., Goldsmith, P., Unson, C. & Spiegel, A. J. biol. Chem. 266, 9309–9313 (1991).

    Article  CAS  Google Scholar 

  21. Gutowski, S. et al. J. biol. Chem. 266, 20519–20524 (1991).

    Article  CAS  Google Scholar 

  22. Ryu, S. H. et al. J. biol. Chem. 265, 17941–17945 (1990).

    Article  CAS  Google Scholar 

  23. Rubins, J. B., Benditt, J. O., Dickey, B. F. & Riedel, N. Proc. natn. Acad. Sci. U.S.A. 87, 7080–7084 (1990).

    Article  ADS  CAS  Google Scholar 

  24. Seydel, U. & Gerace, L. J. biol. Chem. 266, 7602–7608 (1991).

    Article  CAS  Google Scholar 

  25. Michell, R. H. Curr. Biol. 2, 200–202 (1992).

    Article  CAS  Google Scholar 

  26. Payrastre, B. et al. J. biol. Chem. 267, 5078–5084 (1992).

    Article  CAS  Google Scholar 

  27. Heslop, J. P., Blakely, D. M., Brown, K. D., Irvine, R. F. & Berridge, M. J. Cell 47, 703–709 (1986).

    Article  CAS  Google Scholar 

  28. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Institutes of Human Anatomy at the Universitie of Bologna, V. Irnerio, 48, 40126, Bologna, Italy

    Alberto M. Martelli & Lucio Cocco

  2. Institutes of Human Anatomy at the Universitie of Ferrara, V. Fossato di Mortara, 66, 44100, Bologna, Italy

    Valeria Bertagnolo & Luca M. Neri

  3. Institutes of Human Anatomy at the Universitie of Chieti, V. dei Vestini, 66013, Bologna, Italy

    Lucia Manzoli

  4. Institute of Cytomorphology, Consiglio Nazionale delle Ricerche, c/o Istituto Rizzoli, Bologna, 40136, Italy

    Luca M. Neri

  5. Department of Molecular and Cellular Physiology, AFRC, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, CB2 4AT, UK

    R. Stewart Gilmour

Authors
  1. Alberto M. Martelli
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  2. R. Stewart Gilmour
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  4. Luca M. Neri
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  5. Lucia Manzoli
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  6. Lucio Cocco
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Martelli, A., Gilmour, R., Bertagnolo, V. et al. Nuclear localization and signalling activity of phosphoinositidase Cβ in Swiss 3T3 cells. Nature 358, 242–245 (1992). https://doi.org/10.1038/358242a0

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