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Infectious Mononucleosis: Continuous Suspension Culture of Peripheral Blood Leucocytes

Nature volume 217, pages 564–565 (1968)Cite this article

Abstract

THE atypical lymphocyte in patients with infectious mononucleosis, like the leukaemic lymphocyte, is capable of spontaneous in vivo proliferation. This is shown by dividing and binucleate forms in circulation1, increased concentrations of cellular enzymes associated with nucleic acid synthesis2, markedly increased premitotic activity with incorporation of tritiated nucleoside precursors3 and early in vitro division of some of these labelled cells4. Spontaneous lymphoblastoid transformation of fibroblastic elements derived from bone marrow of patients with infectious mononucleosis has been described5 with long term culture of these cells in suspension6. Despite the increased proliferative potential of peripheral atypical lymphocytes in this disease, little is known about the progeny of these cells. No established culture of lymphoid cells derived from peripheral blood of patients with infectious mononucleosis has previously been reported.

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Authors and Affiliations

  1. National Institutes of Health, Bethesda, Maryland

    PHILIP R. GLADE, JULIUS A. KASEL, HAROLD L. MOSES, JACQUELINE WHANG-PENG, PETER F. HOFFMAN, JOHN K. KAMMERMEYER & LAWRENCE N. CHESSIN

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  1. PHILIP R. GLADE
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  2. JULIUS A. KASEL
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  3. HAROLD L. MOSES
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  4. JACQUELINE WHANG-PENG
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  5. PETER F. HOFFMAN
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  6. JOHN K. KAMMERMEYER
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  7. LAWRENCE N. CHESSIN
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GLADE, P., KASEL, J., MOSES, H. et al. Infectious Mononucleosis: Continuous Suspension Culture of Peripheral Blood Leucocytes. Nature 217, 564–565 (1968). https://doi.org/10.1038/217564a0

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