Abstract
FOR quantitative determinations of amino-acids separated on filter paper, it is often desirable that the spots should contain a larger quantity of substance than is as a rule possible to achieve with unaffected resolution. A one-dimensional technique in which the substance to be analysed is applied along a line across the paper has been described by Yanofsky, Wasserman and Bonner1. I have successfully applied the following two-dimensional technique for separating comparatively large quantities of amino-acids in bacterial hydrolysates.
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References
Yanofsky, C., Wasserman, E., and Bonner, D. M., Science, 111, 61 (1950).
Miettinen, J. K., and Virtanen, A. I., Acta Chem. Scand., 3, 459 (1949).
Work, E., Biochimica et Biophysica Acta, 3, 400 (1949).
Woiwod, A. J., Biochem. J., 45, 412 (1949).
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HEDÉN, CG. A Method for Large-scale Separation of Amino-acids on Filter Paper. Nature 166, 999–1000 (1950). https://doi.org/10.1038/166999b0
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DOI: https://doi.org/10.1038/166999b0
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