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A ‘Dry’ Mounting Autoradiographic Technique for the Localization of Water-soluble Compounds

Abstract

ONE of the disadvantages of the stripping film autoradiographic technique is that the film is floated on a water surface and apposed to the radioactive material by dipping the slide into the water and picking up the film so that its under surface (the emulsion layer) is in direct contact with the specimen. During this mounting procedure (called here the wet technique) and subsequent drying period, which may be several hours, there is opportunity for water-soluble compounds to diffuse out of the specimen, or to leave their original site and be absorbed elsewhere1,2.

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References

  1. Doniach, I., and Pelc, S. R., J. Radiol., 23, 184 (1950).

    Article  Google Scholar 

  2. Fitzgerald, P. J., Simmel, E., Weinstein, J., and Martin, C., Lab. Invest., 2, 181 (1953).

    CAS  Google Scholar 

  3. Creasey, W. A., and Stocken, L. A., Biochem. J., 72, 519 (1959).

    Article  CAS  Google Scholar 

  4. Feder, N., and Sidman, R. L., J. Biophys. and Biochem. Cytol., 4, 593 (1958).

    Article  CAS  Google Scholar 

  5. Fitzgerald, P. J., Eidinoff, M. L., Knoll, J. E., and Simmel, E. B., Science, 114, 494 (1951).

    Article  ADS  CAS  Google Scholar 

  6. Pearse, A. G. E., “Histochemistry, Theoretical and Applied”, second ed, (J. and A. Churchill, Ltd., London, 1960).

    Google Scholar 

  7. Coons, A. H., Leduc, E. H., and Kaplan, M. H., J. Exp. Med., 93, 173 (1951).

    Article  CAS  Google Scholar 

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FITZGERALD, P., ORD, M. & STOCKEN, L. A ‘Dry’ Mounting Autoradiographic Technique for the Localization of Water-soluble Compounds. Nature 189, 55–56 (1961). https://doi.org/10.1038/189055a0

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  • DOI: https://doi.org/10.1038/189055a0

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