Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli

Ribosome recycling factor (RRF) catalyzes the disassembly of a termination complex during the final stage of protein synthesis. RRF from Escherichia coli has been crystallized with PEG 400 as precipitant at 287 K. The crystal belongs to the trigonal space group P3₁21 (or P3₂21), with unit-cell parameters a = b = 48.08, c = 141.67 Å. Native data were collected from a frozen crystal to a resolution of 3.0 Å on a Cu Kα rotating-anode X-ray source.