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Determination of oligosaccharides (DP1-DP8) in samples with high content of salt and organic matter by reversed phase HPLC

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Summary

A method is described for the determination of oligosaccharides in samples of high salt content by RP-HPLC with water as eluent and refractive index detection. The samples, mussel processing wastes which are used as media for microfungus and yeast cultures and hence for enzymatic assays (amilolitic capacity) of these microorganisms that develop within the wastes, have been treated with ethanol to precipitate the macromolecular organic matter and desalted by a modified mixed bed resin before chromatographic analysis is carried out.

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Authors and Affiliations

  1. Instituto de Investigaciones Marinas (C.S.I.C.), Muelle de Bouzas s/n., 36208, Vigo, Spain

    J. M. Franco & J. L. Garrido

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  1. J. M. Franco
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  2. J. L. Garrido
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Franco, J.M., Garrido, J.L. Determination of oligosaccharides (DP1-DP8) in samples with high content of salt and organic matter by reversed phase HPLC. Chromatographia 23, 557–560 (1987). https://doi.org/10.1007/BF02324863

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  • DOI: https://doi.org/10.1007/BF02324863

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