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Separation of chlorophylls and their degradation products in marine phytoplankton by reversed-phase high-performance liquid chromatography

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Summary

An RP-HPLC procedure which separates chlorophylls and their degradation products is described. By employing an amonium acetate buffered mobile phase and a linear gradient elution, complex mixtures of chloroand phaeopigments are separated in less than 30 minutes. The method has been applied to the control of chlorophyll enzymatic degradation in cultures of two algal species, and has also been successfully used in the analysis of algal xanthophylls.

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Authors and Affiliations

  1. Instituto de Investigaciones Marinas (C.S.I.C.), Muelle de Bouzas s/n, Vigo-36208, Pontevedra, Spain

    M. Zapata, Ana M. Ayala, J. M. Franco & J. L. Garrido

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  1. M. Zapata
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  2. Ana M. Ayala
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  3. J. M. Franco
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  4. J. L. Garrido
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Zapata, M., Ayala, A.M., Franco, J.M. et al. Separation of chlorophylls and their degradation products in marine phytoplankton by reversed-phase high-performance liquid chromatography. Chromatographia 23, 26–30 (1987). https://doi.org/10.1007/BF02310413

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  • DOI: https://doi.org/10.1007/BF02310413

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