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DNA reassociation kinetics and hybridization in different angiosperms

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Abstract

Reassociation kinetics ofDaucus carota andPetroselinum crispum (Apiaceae), andDatura innoxia (Solanaceae) are presented. Hybridization of3H-labelled DNA of two carrot cultivars indicate strong qualitative homologies of DNA sequences; nevertheless, certain quantitative differences in some Cotregions seem to exist. However, homologous sequences ofDaucus DNA with DNA ofDatura, and, suprisingly, even with DNA ofPetroselinum are very restricted: between 8% in the repeated regions and ca. 7–9% in the unique regions.

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References

  • Britten, R. J., Kohne, D. E., 1968: Repeated sequences in DNA. — Science161, 529–540.

    Google Scholar 

  • Davidson, E. H., Hough, B. R., Amenson, C. S., Britten, R. J., 1973: General interspersion of repetitive with non-repetitive sequence elements in the DNA ofXenopus. — J. Mol. Biol.77, 1–23.

    Google Scholar 

  • Dührssen, E., 1980: Untersuchungen zur Charakterisierung der Satelliten-DNS pflanzlicher Gewebekulturen (Daucus carota L.,Datura innoxia Mill.). (In preparation).

  • -Neumann, K.-H., 1980: Characterization of satellite-DNA ofDaucus carota L. — Z. Pflanzenphysiol. (in print).

  • Gould, A. R., Bayliss, M. W., Street, H. E., 1974: Studies on the growth in culture of plant cells. — J. Exp. Bot.25, 468–478.

    Google Scholar 

  • Jeannin, G., Melet, D., Kovoor, A., 1972: Differential extraction of nucleic acids from large quantities of plant tissue: DNA and transfer RNA from Jerusalem Artichoke rhizomes. — J. Exp. Bot.23, 863–874.

    Google Scholar 

  • Jaworski, A., Key, J. L., 1974: Distribution of ribosomal deoxyribonucleic acid in subcellular fractions of higher plants. — Plant Physiol.53, 366–369.

    Google Scholar 

  • Laird, D. D., 1971: Chromatid structure: relationship between DNA content and nucleotide sequence diversity. — Chromosoma32, 378–406.

    Google Scholar 

  • Mitra, R., Bhatia, C. R., 1973: Repeated and non-repeated nucleotide sequences in diploid and polyploid wheat species. — Heredity31, 251–262.

    Google Scholar 

  • Neumann, K.-H., 1966: Wurzelbildung und Nukleinsäuregehalt bei Phloem-Gewebekulturen der Karottenwurzel auf synthetischen Nährmedien verschiedener Hormonkombinationen. — Les phytohormones et l'organogénèse, Les congrès et collogues de l'université de Liège,38, 95–102.

    Google Scholar 

  • Nze-Ekekang, L., Pattilon, M., Schäfer, A., Kovoor, A., 1974: Repetitive DNA of higher plants. Separation of fractions of different complexity from Jerusalem Artichoke tissue by reassociation kinetics. — J. Exp. Bot.25, 320–329.

    Google Scholar 

  • Schäfer, A., Neumann, K.-H., 1978: The influence of Gibberellic acid on reassociation kinetics of DNA ofDaucus carota L. — Planta143, 1–4.

    Google Scholar 

  • Smith, D. B., Flavell, R. B., 1974: The relateness and evolution of repeated nucleotide sequences in the genomes of someGramineae species. — Biochem. Genet.12, 243–256.

    Google Scholar 

  • Stein, D. B., Thompson, W. F., 1975: DNA hybridization and evolutionary relationship in threeOsmunda species. — Science189, 888–889.

    Google Scholar 

  • Wetmur, J., Davidson, N., 1968: Kinetics of renaturation of DNA. — J. Mol. Biol.31, 349–370.

    Google Scholar 

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Dührssen, E., Saavedra, E. & Neumann, K.H. DNA reassociation kinetics and hybridization in different angiosperms. Pl Syst Evol 136, 267–273 (1980). https://doi.org/10.1007/BF01004631

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