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On the estimation of alternative pathways of fatty acid oxidation in the liverIn vivo

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Abstract

The relative contributions of mitochondrial β-oxidation and peroxisomal β-oxidation and peroxisomal ω-oxidation to the oxidation of a given fatty acidin vivo can be quantitated by an isotopic method. The approach requires infusion of a fatty acid labelled on two specific carbon atoms (e.g. [1-14C] and [11-14C] palmitate) to an isotopic steady state, with subsequent isolation and degradation of an acetylated conjugate as a product of the liver cytosolic acetyl CoA pool and of ketone bodies as a product of the liver mitochondrial acetyl CoA pool.

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  1. Whittier Institute, 9894 Genesee Avenue, 92037, La Jolla, CA, U.S.A.

    Robert Rognstad

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Rognstad, R. On the estimation of alternative pathways of fatty acid oxidation in the liverIn vivo . Bltn Mathcal Biology 57, 191–203 (1995). https://doi.org/10.1007/BF02460615

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