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ATP synthase subunit c/III/9 gene sequences as a tool for interkingdom and metaphytes molecular phylogenies

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Summary

The 38 sequences of the ATPase c/III/9 gene determined in bacteria, fungi, mammals, and higher plants have been used to construct phylogenetic trees by distance matrix and parsimony methods (checked by bootstrapping); alignments have been performed on the deduced amino-acid sequences and then transferred back to the nucleotide sequences. Three lineages stand out: (1) eubacteria (except cyanobacteria and a purple bacteria), (2) chloroplasts, together with cyanobacteria, and (3) mitochondria together with nuclei and α purple bacteria. The clear monophyly of the mitochondrial/nuclear lineage, taken all together, strongly suggests that the nuclear copies of the gene now residing in the eukaryotic nucleus originate from a mitochondrial transfer. Within this lineage, metaphytes emerge late and as a cohesive group, after fungi (as a dispersed group) and metazoa, yielding an order that markedly differs from that obtained through typical RNA nuclear molecules. The possible biphyletic origin of mitochondria based on mitochondrial rRNA sequences is not evidenced by these sequences. Internal branches within both the chloroplastic and the mitochondrial lineages are consistent with botanical evolutionary schemes based on morphological characters. In spite of its relatively small size, the ATPase c/III/9 gene therefore displays remarkable properties as a phylogenetic index and adds a new tool for molecular evolutionary reconstructions, especially within the metaphytes.

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  1. Hervé Recipon

    Present address: National Center for Biotechnology Information, National Library of Medicine, Bldg 38 A, NIH, 8600 Rockville Pike, 20894, Bethesda, MD, USA

Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire Végétale, Université Paris XI, URA CNRS D1128, Bât 430, 91405, Orsay Cedex, France

    Hervé Recipon & Francis Quetier

  2. Laboratoire de Biologie Cellulaire 4, Université Paris XI, URA CNRS 1134, Bât 444, 91405, Orsay Cedex, France

    Roland Perasso & André Adoutte

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  1. Hervé Recipon
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Recipon, H., Perasso, R., Adoutte, A. et al. ATP synthase subunit c/III/9 gene sequences as a tool for interkingdom and metaphytes molecular phylogenies. J Mol Evol 34, 292–303 (1992). https://doi.org/10.1007/BF00160236

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