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Differences in amino acid sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from two species of Dasycladaceae

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Abstract

In contrast to other plants the plastid genome of Acetabularia is larger in size and shows a high degree of variability. This study on the chloroplast-encoded large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase demonstrates that strongly conserved areas also exist in the plastid genome of the Dasycladaceae. Searching for differences in the amino acid sequence of the large subunit from Acetabularia mediterranea and Acicularia schenckii, proteolytic peptides which differ in their elution behaviour in reverse-phase high-performance liquid chromatography were sequenced. Only six amino acids were found to be exchanged in the large subunit from these two species. Since these two species diverged approx. 150 million years ago, these results imply that 0.84 amino-acid exchanges per 100 amino acids have occurred in 108 years, underlining the strong conservatism of the large subunit.

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Abbreviations

A:

Acetabularia mediterranea

Ac.:

Acicularia schenckii

HPLC:

high-performance liquid chromatography

LSU:

large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase

PAGE:

polyacrylamide gel electrophoresis

RuBPCase:

ribulose-1,5-bisphosphate carboxylase/oxygenase

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Max-Planck-Institut für Zellbiologie, D-6802, Ladenburg, Federal Republic of Germany

    M. B. Leible & H. -G. Schweiger

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  1. M. B. Leible
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  2. H. -G. Schweiger
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Leible, M.B., Schweiger, H.G. Differences in amino acid sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from two species of Dasycladaceae. Planta 169, 575–582 (1986). https://doi.org/10.1007/BF00392109

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  • DOI: https://doi.org/10.1007/BF00392109

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