Abstract
In order to elucidate the NAD-recycling pathway the following enzyme activities have been characterized in different tobacco tissues and in tomato root: NAD pyrophosphatase, nicotinamide mononucleotide (NMN)/nicotinic acid mononucleotide (NaMN) glycohydrolases, nicotinamidase and nicotinic acid phosphoribosyltransferase. The investigations were performed with protein extracts purified by gel filtration and enzymatic activities were determined by high-performance liquid chromatography methods. The kinetic parameters of the different enzymes from tobacco root and their specificity are reported. The data are in favor of the so-called pyridine-nucleotide cycle VI (NAD→NMN→nicotinamide→nicotinic acid→NaMN→nicotinic acid adenine dinucleotide→NAD). In the nicotine-producing tobacco root a further direct route leading from NaMN to nicotinic acid is proposed. These data are reconciled with the assumption that it is nicotinic acid which is provided by the pyridine-nucleotide cycle for the synthesis of nicotine.
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Abbreviations
- HPLC:
-
high-performance liquid chromatography
- Na:
-
nicotinic acid
- NaAD:
-
nicotinic acid adenine dinucleotide
- NaMN:
-
nicotinic acid mononucleotide
- NMN:
-
nicotinamide mononucleotide
- PRPP:
-
5-phosphoribosyl-1-pyrophosphate
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This contribution is dedicated to Professor Augustin Betz on the occasion of his 65th birthday
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Wagner, R., Feth, F. & Wagner, K.G. The pyridine-nucleotide cycle in tobacco. Planta 167, 226–232 (1986). https://doi.org/10.1007/BF00391419
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DOI: https://doi.org/10.1007/BF00391419