Summary
Somatic embryogenesis from different genotypes of Asparagus officinalis L. could be obtained by in vitro culture of shoot apices. Apices were first cultured on an auxin-rich inducing medium and then transferred onto a hormone-free development medium. All genotypes tested in this way produced a few somatic embryos. In some experiments, during the development phase, a new kind of friable highly embryogenic tissue appeared in a random manner. These tissues could be continuously subcultured on a hormone-free medium and were named embryogenic lines. Five of these embryogenic lines regenerated plants from somatic embryos. These regenerated plants exhibited an increased embryogenic response compared to the parent plants; e.g. apex culture produced somatic embryos without any auxin treatments. For one of the embryogenic lines, a genetic analysis showed that the improved embryogenic response of regenerated plants was controlled by a mendelian dominant monogenic mutation.
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Abbreviations
- LSEA:
-
low somatic embryogenesis ability
- HSEA:
-
high somatic embryogenesis ability
- NAA:
-
1-naphthaleneacetic acid
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Delbreil, B., Jullien, M. Evidence for in vitro induced mutation which improves somatic embryogenesis in Asparagus officinalis L.. Plant Cell Reports 13, 372–376 (1994). https://doi.org/10.1007/BF00234140
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DOI: https://doi.org/10.1007/BF00234140