Summary
Somatic hybrid plants were recovered following fusion of leaf mesophyll protoplasts isolated from tomato (Lycopersicon esculentum) cultivar UC82 with protoplasts isolated from suspension cultured cells of L. chilense, LA 1959. Iodoacetate was used to select against the growth of unfused tomato protoplasts. Two somatic hybrids were recovered in a population of 16 regenerants. No tomato regenerants were recovered; all of the non-hybrid regenerants were L. chilense. The L. chilense protoplast regenerants were tetraploid. The hybrid nature of the plants was verified using species-specific restriction fragment length polymorphisms for the nuclear, chloroplast and mitochondrial genomes. The somatic hybrids had inherited the chloroplast DNA of the tomato parent, and portions of the mitochondrial DNA of the L. chilense parent. The somatic hybrids formed flowers and developed seedless fruit.
Similar content being viewed by others
References
Austin S, Baer MA, Helgeson JP (1985) Plant Sci 39:75–82
Bonnema AB, Melzer JM, O'Connell MA (1991) Theor Appl Genet 81:339–348
Doyle JF, Doyle JL (1989) Focus 12:13–14
Kemble RJ, Barsby TL, Yarrow SA (1988) Mol Gen Genet 213:202–205
Kinsara A, Patnaik SN, Cocking EC, Power JB (1986) Plant Physiol 125:225–234
Kyozuka J, Kaneda T, Shimamoto K (1989) Biotech 7:1171–1174
Melzer JM, O'Connell MA (1990) Theor Appl Genet 79:193–200
Melzer JM, O'Connell MA (1991) Theor Appl Genet (in press)
Morgan A, Maliga P (1987) Mol Gen Genet 209:240–246
Mutschler MA, Tanksley SD, Rick CM (1987) Tomato Genet Coop Rep 37:5–34
O'Connell MA, Hanson MR (1985) Theor Appl Genet 70:1–12
O'Connell MA, Hanson MR (1986) Theor Appl Genet 72:59–65
O'Connell MA, Hanson MR (1987) Theor Appl Genet 75:83–89
Rick CM (1973) In: Srb AM (ed) Genes, Enzymes and Populations. Plenum Press, New York, pp 255–269
Rick CM (1979) In: Hawkes JG, Lester RN, Skelding AD (eds) The Biology and Taxonomy of the Solanaceae. Academic Press, London, pp 667–678
San LH, Vedel F, Sihachakr D, Remy R (1990) Mol Gen Genet 221:17–26
Sjodin C, Glimelius K (1989) Theor Appl Genet 78:513–520
Wijbrandi J, Zabel P, Koornneef M (1990) Mol Gen Genet 222:270–277
Zamir D, Tanksley SD (1988) Mol Gen Genet 213:254–261
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Bonnema, A.B., O'Connell, M.A. Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) and Lycopersicon chilense . Plant Cell Reports 10, 629–632 (1992). https://doi.org/10.1007/BF00232385
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232385