Summary
The psbA gene is part of the reaction center of photosystem II in cyanobacteria and the plastids of higher plants. Its primary sequence is highly conserved among all species investigated so far and its sequence shows homologies with the L and M subunits of the reaction center of photosynthetic bacteria. We have analyzed the psbA homolog from a eukaryotic alga, Cyanophora paradoxa, where the gene is encoded on cyanelle DNA. These cyanelles are surrounded by a murein sacculus and resemble cyanobacteria in many other characteristics, although they are genuine organelles that functionally replace plastids. Analysis of the gene revealed a psbA protein identical in length (360 codons) with the cyanobacterial counterpart. The overall sequence identity is, however, more pronounced between cyanelle psbA and the shorter (353 amino acids) psbA product found in higher plants. These data strongly support the postulated bridge position of cyanelles between chloroplasts and free-living cyanobacteria.
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References
Aitken A, Stanier RY (1979) J Gen Microbiol 112:219–223
Berenguer J, Rojo F, de Pedro MA, Pfanzagl B, Löffelhardt W (1987) FEBS Lett 224(2):401–405
Barber J (1987) Trends Biochem Sci 12:123–124
Birnboim C, Doly J (1979) Nucl Acids Res 7:1513–1523
Bohnert HJ, Michalowski C, Bevacqua S, Mucke H, Löffelhardt W (1985) Mol Gen Genet 201:565–574
Breiteneder H, Seiser C, Löffelhardt W, Michalowski C, Bohnert HJ (1988) Curr Genet 13:199–206
Brusslan J, Haselkorn R (1988) Photosynthesis Res 17:115–124
Chen EY, Seeburg PH (1985) DNA 4:165–170
Chisholm D, Williams JGK (1988) Plant Mol Biol 10:293–301
Curtis SE, Haselkorn R (1984) Plant Mol Biol 3:249–258
Debus RJ, Barry BA, Babcock GT, McIntosh L (1988) Proc Nbatl Acad Sci USA 85:427–430
Deisenhofer J, Epp O, Miki K, Huber R, Michel H (1985) Nature 318:618–624
Erickson JM, Rahire M, Rochaix JD (1984) EMBO J 3(12):2753–2762
Evrard JL, Kuntz M, Straus NA, Weil JH (1988) Gene 71(l):115–122
Geliebter J (1987) FOCUS 9(l):5–8
Giovannoni SJ, Turner S, Olsen GJ, Barns S, Lane DJ, Pace NR (1988) J Bacteriol 170:3584–3592
Golden SS, Brusslan J, Haselkorn R (1986) EMBO J 5(11):2789–2798
Hanley-Bowdoin L, Chua NH (1987) TIBS 12:67–70
Hanley-Bowdoin L, Chua NH (1988) Plant Mol Biol 10:303–310
Hanley DK, McClure WR (1983) Nucleid Acids Res 11:2237–2255
Janssen I, Mucke H, Löffelhardt W, Bohnert HJ (1987) Plant Mol Biol 9:479–484
Janssen I (1988) Thesis. University of Vienna
Karabin GD, Farley M, Hallick RB (1984) Nucleic Acid Res 12 (14):5801–5812
Keller M, Stutz E (1984) FEBS Lett 175(1):173–177
Ko K, Jaynes JM, Straus NA (1985) Plant Sci 42:115–123
Lambert DH, Bryant DA, Stirewalt VL, Dubbs JM, Stevens SE, Porter RD (1985) J Bacteriol 164:659–664
Lemaux PG, Grossman AR (1985) EMBO J 4:1911–1919
Löffelhardt W, Mucke H, Crouse EJ, Bohnert HJ (1983) Curr Genet 7:139–144
Markowicz Y, Mache R, Loiseaux-de Goer S (1988) Plant Mol Biol 10:465–469
Maxwell EX, Liu J, Shively JM (1986) J Mol Evol 23:300–304
Morden CW, Golden SS (1989) Nature 337:382–385
Mulligan B, Schultes N, Chen L, Bogorad L (1984) Proc Natl Acad Sci USA 81:2693–2697
Nanba O, Satoh K (1987) Proc Natl Acad Sci USA 84:109–112
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–304
Reith M, Cattolico RA (1986) Proc Natl Acad Sci USA 83:8599–8603
Sherman L, Bricker T, Guikema J, Pakrasi H (1987) The protein composition of the photosynthetic complexes from the cyanobacterial thylakoid membrane. In: Fay P, VanBaalen C (eds) The cyanobacteria. Elsevier, Amsterdam New York, p 1–34
Starnes SM, Lambert DH, Maxwell ES, Stevens SE Jr, Porter RD, Shively JM (1985) FEMS Microbiol Lett 28:165–169
Stern DB, Gruissem W (1987) Cell 51:1145–1157
Thomas PS (1983) Proc Natl Acad Sci USA 77:5201–5205
Trebst A (1986) Z Naturforschung 41C:240–245
Wasmann CC, Löffelhardt W, Bohnert HJ (1987) Cyanelles: organization and molecular biology. In: Fay P, Van Baalen C (eds) The cyanobacteria. Elsevier, Amsterdam New York, p 303–324
Wu NH, Cote JC, Wu R (1987) Dev Genetics 8:339–350
Zurawski G, Bohnert HJ, Whitfeld PR, Bottomley W (1982) Proc Natl Acad Sci USA 79:7699–7703
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Janssen, I., Jakowitsch, J., Michalowski, C.B. et al. Evolutionary relationship of psbA genes from cyanobacteria, cyanelles and plastids. Curr Genet 15, 335–340 (1989). https://doi.org/10.1007/BF00419913
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DOI: https://doi.org/10.1007/BF00419913