Summary
We determined the restriction maps and primary structures of two as yet poorly characterized regions of the mitochondrial genomes of different wildtype strains of Saccharomyces cerevisiae. These regions respectively comprised the ori1 sequence and the newly identified ori8 sequence. Ori1 and ori8, together with their flanking sequences, exhibit a large polymorphism, resulting from specific variations due to insertions or deletions of optional GC clusters at different locations. The mechanisms underlying such sequence rearrangements are discussed.
Similar content being viewed by others
References
Baldacci G, Bernardi G (1982) EMBO J 1:987–994
Baldacci G, Bernardi G (1983) The replication of yeast mitochondrial DNA. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983. de Gruyter, Berlin, pp 57–63
Baldacci G, Chérif-Zahar B, Bernardi G (1984) EMBO J 3:2115–2120
Bernardi G, Prunell A, Kopecka H (1975) An analysis of the mitochondrial genome of yeast with restriction enzymes. In: Puiseux-Dao S (ed) Molecular biology of nucleocytoplasmic relationships. Elsevier, Amsterdam, pp 84–90
Blanc H, Dujon B (1980) Proc Natl Acad Sci USA 77:3942–3946
Blanc H, Dujon B (1982) Replicator regions of the yeast mitochondrial DNA active in vivo and in yeast transformants. In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 279–294
Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A (1980) J Biol Chem 255:11922–11926
Christianson T, Edwards JC, Levens D, Locker J, Rabinowitz M (1982) J Biol Chem 257:6494–6500
Christianson T, Rabinowitz M (1983) J Biol Chem 258:14025–14033
Clayton DA (1982) Cell 28:693–705
Cobon GS, Beilharz MW, Linnane AW, Nagley P (1982) Curr Genet 5:97–107
Colin Y, Baldacci G, Bernardi G (1985) Gene 36:1–13
Coruzzi G, Bonitz SG, Thalenfeld BE, Tzagoloff A (1981) J Biol Chem 256:12780–12787
de Zamaroczy M, Marotta R, Faugeron-Fonty G, Goursot R, Mangin M, Baldacci G, Bernardi G (1981) Nature (London) 292:75–78
de Zamaroczy M, Faugeron-Fonty G, Bernardi G (1983) Gene 51:193–202
de Zamaroczy M, Faugeron-Fonty G, Baldacci G, Goursot R, Bernardi G (1984) Gene 32:439–457
Dujon B (1980) Cell 20:185–197
Faugeron-Fonty G, Culard F, Baldacci G, Goursot R, Prunell A, Bernardi G (1979) J Mol Biol 134:493–537
Faugeron-Fonty G, Mangin M, Huyard A, Bernardi G (1983) Gene 24:61–71
Faugeron-Fonty G, Le Van Kim C, de Zamaroczy M, Goursot R, Bernardi G (1984) Gene 32:459–473
Fonty G, Goursot R, Wilkie D, Bernardi G (1978) J Mol Biol 119:213–235
Gaillard C, Bernardi G (1979) Mol Gen Genet 174:335–337
Gaillard C, Strauss F, Bernardi G (1980) Nature (London) 283:218–220
Goursot R, Mangin M, Bernardi G (1982) EMBO J 1:705–711
Goyon C (1984) Contribution à l'étude des remaniements dans les séquences ori du génome mitochondrial de la levure. Diplôme d'Etudes Approfondies, Univ. Paris VI
Hensgens LAM, Arnberg AC, Roosendaal E, Van den Horst G, Van den Veen R, Van Ommen G-JB, Grivell LA (1983) J Mol Biol 164:35–38
Hudspeth MES, Vincent RD, Perlman PS, Shumard SD, Treisman LO, Grossman LI (1984) Proc Natl Acad Sci USA 81:3148–3152
Le Van Kim C (1981) Contribution à l'étude de la suppressivíté chez la levure Saccharomyces cerevisiae. Diplôme d'Etudes Approfondies, Univ. Paris VII
Mahler HR (1983) Int Rev Cytol 82:1–98
Mangin M, Faugeron-Fonty G, Bernardi G (1983) Gene 24:73–81
Martin RP, Bordonné R, Dirheimer G (1982) The paromomycin region in the yeast mitochondrial genome. In: Akoyounoglou G, Evangelopoulos EA, Georgatsos J, Palaiologos G, Trakatellis A, Tsiganos CP (eds) Cell function and differentiation, part B. Liss, New York, pp 355–365
Messing J, Vieira J (1982) Gene 19:269–276
Nobrega FG, Tzagoloff A (1980) J Biol Chem 255:9828–9837
Osinga KA, Tabak HF (1982) Nucleic Acids Res 10:3617–3626
Osinga KA, De Vries E, Van der Horst GTJ, Tabak HF (1984) Nucleic Acids Res 12:1889–1900
Prunell A, Bernardi G (1977) J Mol Biol 110:53–74
Prunell A, Kopecka H, Strauss F, Bernardi G (1977) J Mol Biol 110:17–52
Sanders JPM, Heyting C, Verbeert MP, Meijlink FCPW, Borst P (1977) Mol Gen Genet 157:239–261
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sor F, Fukuhara H (1982a) Nucleic Acids Res 10:1625–1633
Sor F, Fukuhara H (1982b) Nucleotide sequence of the small ribosomal RNA gene from the mitochondria of Saccharomyces cerevisiae. In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 255–262
Sor F, Fukuhara H (1983) Nucleic Acids Res 11:339–348
Wolf K, Dujon B, Slonimski PP (1973) Mol Gen Genet 125:53–90
Zassenhaus HP, Butow R (1983) Functions of non-genic DNA in yeast mitochondria. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983. De Gruyter, Berlin, pp 95–106
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Faugeron-Fonty, G., Goyon, C. Polymorphic variations in the ori sequences from the mitochondrial genomes of different wild-type yeast strains. Curr Genet 10, 269–282 (1985). https://doi.org/10.1007/BF00365623
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365623