Summary
Giant protoplasts of Pleurotus cornucopiae were fused, using the glass microelectrode electrofusion technque; the percentage fusion achieved was 70%. To induce fusion, Ca2+ was necessary, a 10 mM concentration giving the best result. Polyethylene glycol 4000 (PEG) promoted fusion but also increased the adhesion of protoplasts, which caused them to be irreversibly attached to the electrodes. Fusion was always completed within 1 min after a single electrical pulse had been applied. The fused protoplast was isolated with a glass micropipette and was found to regenerate into a colony.
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References
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Magae, Y., Kashiwagi, Y., Senda, M. et al. Electrofusion of giant protoplasts of Pleurotus cornucopiae . Appl Microbiol Biotechnol 24, 509–511 (1986). https://doi.org/10.1007/BF00250332
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DOI: https://doi.org/10.1007/BF00250332