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Transformation of LMTK− cells with purified HLA class I genes

II. Serologic characterization of HLA-A3 and CW3 molecules

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Abstract

The expression of two different HLA class I genes was observed after transformation of LMTK− cells. The corresponding class I molecules reacted differentially with monomorphic monoclonal antibodies (m.Ab). Absorption and elution studies of the human alloantibodies reacting with the transformed cells and cellular radioimmunoassay of these cells with polymorphic m.Ab resulted in the identification of HLA-A3 and CW3 molecules. These transformed cells were used to immunize C3H mice and induce the production of xenogeneic antisera, which, following absorption, showed polymorphic reactivity with human cells, suggesting that some of these sera could be used as typing reagents.

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Abbreviations

β2-m:

β2-m-microglobulin

EBV:

Epstein Barr virus

FCS:

fetal calf serum

Hepes:

N-2-hydroxypiperazine-N′-2-ethane sulfonic acid

m.Ab:

monoclonal antibody

PBL:

peripheral blood lymphocytes

PMSF:

phenylmethanesulfonyl fluoride

SDS:

sodium dodecyl sulfate

TRIS:

trishydroxymethyl-aminomethane

References

  • Barbosa, J. A., Kamarck, M. E., Biro, P. A., Weissman, S. M., and Ruddle, F. H.: Identification of human genomic clones coding the major histocompatibility antigens HLA-A2 and HLA-B7 by DNAmediated gene transfer. Proc. Natl. Acad. Sci. U. S. A. 79: 6327–6331, 1982

    PubMed  Google Scholar 

  • Barnstable, C. J., Bodmer, W. F., Brown, G., Galfre, G., Milstein, C., Williams, A. F., and Ziegler, A.: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens. New tools for genetic analysis. Cell 14: 9–20. 1978

    Article  Google Scholar 

  • Beverley, P.: Production and use of monoclonal antibodies in transplantation immunology. In J. L. Tourraine, G., Traeger, H. Betuil, G. Brochier, J. M. Dubernard, J. P. Revillard, and R. Triau (eds.): Transplantation and Clinical Immunology, pp. 87–94, Excerpta Medica, Amsterdam, 1980

    Google Scholar 

  • Biddisson, W. E., Krangel, M. S., Strominger, J. L., Ward, F. E., Shearer, G. M., and Shaw, S.: Virusimmune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. Hum. Immunol. 3: 225–232, 1980.

    Article  Google Scholar 

  • Biddisson, W. E., Shearer, G. M., and Shaw, S.: Influenza virus-specific cytotoxic T cells are restricted by multiple HLA-A3-related self antigens: evidence for recognition of distinct self structures in conjunction with different foreign antigens. J. Immunol. 127: 2231–2235, 1981

    Google Scholar 

  • Billing, R., Clark, B., Lucero, K., Shi, B.J., and Terasaki, P.: A monoclonal antibody recognizing a determinant common to HLA-A3 and A.11. Tissue Antigens 19:121–128, 1982

    PubMed  Google Scholar 

  • Billing, R. and Lucero, K.: A monoclonal antibody against an epitope common to HLA-B locus antigens. Hum. Immunol. 4: 351–357, 1982

    Article  PubMed  Google Scholar 

  • Bodmer, J., Coukell, A., Bodmer, W. F., Payne, R., and Shanbrom, E.: A new allele for the LA series of HL-A antigens: The analysis of a complex serum. In P.I. Terasky, R. Ceffellini, J. J. van Rood, and D.B. Amos (eds.): Histocompatibility Testing, pp. 175–185, Munksgaard, Copenhagen, 1970

    Google Scholar 

  • Bourel, D., Fauchet, R., Genetet, B., Merdrignac, G., and Hurez, D.: A monoclonal antibody to HLA-A3 alloantigens. Immunol. Letters, in press, 1983

  • Brodsky, F.M., Parham, P., Barnstable, C.J., Crumpton, M.J., and Bodmer, W.F.: Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3–61, 1979

    PubMed  Google Scholar 

  • Brodsky, F. M. and Parham, P.: Monomorphic anti-HLA-A, B, C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129–135, 1982

    PubMed  Google Scholar 

  • Dosseto, M., Rohner, C., Pierres, M., and Goridis, C.: Biosynthetic incorporation of (75Se) selenomethionine: a new method for labeling lymphocyte membrane antigens. J. Immunol. Methods 41: 213–224, 1981

    Article  Google Scholar 

  • Festenstein, H. and Démant, P.: The human major histocompatibility system. In J. Turk (ed.): HLA and H-2 Basic Immunogenetics, Biology and Clinical Relevance, pp. 16–89, Edward Arnold Ltd., London, 1978

    Google Scholar 

  • Goding, J. W.: Use of staphylococcal protein A as an immunological reagent. J. Immunol. Methods 20: 241–253, 1978

    Article  PubMed  Google Scholar 

  • Goodenow, R. S., McMillan, M., Orn, A., Nicholson, M., Davidson, N., Frelinger, J. A., and Hood, L.: Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. Science 215: 677–679, 1982

    PubMed  Google Scholar 

  • Hubbard, A. L. and Cohn, Z. A.: Externally disposed plasma membrane proteins. I. Enzymatic iodination of mouse L cells. J. Cell. Biol. 64: 438–460, 1975

    Article  PubMed  Google Scholar 

  • Iványi, P.: Interspecies MHS relationship studied by serological and cellular cross-reactions. In R. A. Reisfield and S. Ferrone (eds.): Current Trends in Histocompatibility, pp. 133–181, Plenum Press, New York, 1981

    Google Scholar 

  • Kalil, J., Lepage, V., Fellous, M., and Colombani, J.: Anticorps monoclonaux anti-HLA. Bull. Inst. Pasteur 80:97–126, 1982

    Google Scholar 

  • Lemonnier, F., Neauport-Sautes, C., Kourilsky, F. M., and Démant, P.: Relationship between private and public H-2 specificities on the cell surface. Immunogenetics 2: 517–529, 1975

    Google Scholar 

  • Lemonnier, F. A., Malissen, M., Golstein, P., Le Bouteiller, P., Rebai, N., Damotte, M., Birnbaum, D., Caillol, D., Trucy, J., and Jordan, B. R.: Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L-cells. Immunogenetics 16:355–361, 1982

    PubMed  Google Scholar 

  • Lemonnier, F. A., Rebai, N., Le Bouteiller, P. P., Malissen, B., Golstein, P., Malissen, M., Mishal, Z., Caillol, D. H., Jordan, B. R., and Kourilsky, F. M.: Transformation of murine LMTK− cells with purified HLA class I genes. I. Modification of conformation of murine β2-microglobulin upon its association with HLA heavy chains. J. Immunol. 130: 1432–1438, 1983

    PubMed  Google Scholar 

  • Liabeuf, A., LeBorgne de Kaouel, C., Kourilsky, F. M., Malissen, B., Manuel, Y., and Sanderson, A. R.: An antigenic determinant of human β2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J. Immunol. 127: 1542–1548, 1981

    PubMed  Google Scholar 

  • Malissen, B., Charmot, D., and Mawas, C.: Expansion of human lymphocyte populations expressing specific immune reactivities.III. Specific colonies, either cytotoxic or proliferative, obtained from a population of responder cells primed in vitro. Preliminary immunogenetic analysis. Hum. Immunol. 2: 1–13, 1981a

    Article  PubMed  Google Scholar 

  • Malissen B., Kristensen, T., Goridis, C., Madsen, M., and Mawas, C.: Clones of human cytotoxic T lymphocytes derived from an allosensitized individual: HLA specificity and cell surface markers. Scand. J. Immunol. 14: 213–224, 1981b

    PubMed  Google Scholar 

  • Malissen, B., Rebai, N., Liabeuf, A., and Mawas, C.: Human cytotoxic T cell structures associated with expression of cytolysis. I. Analysis at the clonal level of the cytolysis inhibiting effect of 7 monoclonal antibodies. Eur. J. Immunol. 9: 739–746, 1982a

    Google Scholar 

  • Malissen, M., Damotte, M., Birnbaum, D., Trucy, J., and Jordan, B. R.: HLA cosmid clones show complete, widely-spaced human class I genes with occasional clusters. Gene 20: 485–489, 1982b

    Article  PubMed  Google Scholar 

  • Oi, V. T., Jones, P. P., Goding, J. N., Herzenberg, L. A., and Herzenberg, L. A.: Properties of monoclonal antibodies to mouse Ig allotypes, H-2 and la antigens, In F. Melchers, M. Potter, and N. L. Warner (eds.): Current Topics in Microbiology and Immunology, pp. 115–125, Springer-Verlag, Berlin, 1978

    Google Scholar 

  • Rebai, N. and Malissen, B.: Structural and genetic analysis of HLA class I molecules using monoclonal xenoantibodies. Tissue Antigens, in press, I983

  • Rebai, N., Malissen, B., Pierres, M., Accolla, R. S., Corte, G., and Mawas, C.: Distinct HLA-DR epitopes and distinct families of HLA-DR molecules defined by 15 m.Ab either anti-DR or allo-anti-Iak crossreacting with human DR molecules. I. Cross-inhibition studies of m.Ab cell surface fixation and differential binding of m.Ab to detergent-solubilized HLA-molecules immobilized to a solid phase by a first m.Ab. Eur. J. Immunol., 13: 106–111, 1983

    PubMed  Google Scholar 

  • Schulman, N. R., Marder, V. J., Hiller, M. C., and Collier, E. M.: Platelet and leukocyte iso-antigens and their antibodies: serologic, physiologic and clinical studies. In C. V. Moore and E. B. Brown (eds.): Progress in Hematology, pp. 222–304, Grune and Stratton, New York and London, 1964

    Google Scholar 

  • Singer, D. S., Camerini-Otero, R. D.,Satz, M. L., Osborne, B., Sachs, D., andRudikoff, S.: Characterization of a porcine genomic clone encoding a major histocompatibility antigens, expression in mouse L cells. Proc. Natl. Acad. Sci. U.S.A. 79: 1403–1407, 1982

    PubMed  Google Scholar 

  • Terasaki, P. (ed.): Histocompatibility Testing 1980, UCLA Tissue Typing Laboratory, Los Angeles, California, 1980

    Google Scholar 

  • Trucco, M. D., Garotta, G., Stocker, J. N., and Ceppellini, R.: Murine monoclonal antibodies against HLA structures. Immunol. Rev. 47: 219–252, 1979

    PubMed  Google Scholar 

  • Walford, R. L., Smith, G. S., and Waters, H.: Histocompatibility systems and disease states with particular reference to cancer. Transplant. Rev. 7: 78–111, 1971

    PubMed  Google Scholar 

  • Wigler, M., Silverstein, S., Leu, L. S., Pellicer, A., Cheng, Y. C., and Axel, R.: Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11: 223–232, 1977

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, case 906, 13288, Marseille Cedex 9, France

    François A. Lemonnier, Patrice C. Dubreuil, Corinne Layet, Marie Malissen, Danielle H. Caillol, François M. Kourilsky & Bertrand R. Jordan

  2. Centre Régional de Transfusion Sanguine, rue Pierre Jean Gineste, 35000, Rennes, France

    Dominique Bourel

  3. Centre de Transfusion Sanguine, 147, boulevard Baille, 13005, Marseille, France

    Pierre Mercier

  4. Tissue Typing Laboratory of the Department of Clinical Immunology, State University Hospital of Copenhagen (Rigshospitalet), Tagensvej 20, DK-2200, Copenhagen N, Denmark

    Bodil K. Jakobsen & Arne Svejgaard

Authors
  1. François A. Lemonnier
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  2. Patrice C. Dubreuil
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  3. Corinne Layet
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  4. Marie Malissen
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  5. Dominique Bourel
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  6. Pierre Mercier
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  7. Bodil K. Jakobsen
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  8. Danielle H. Caillol
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  9. Arne Svejgaard
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  10. François M. Kourilsky
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  11. Bertrand R. Jordan
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Lemonnier, F.A., Dubreuil, P.C., Layet, C. et al. Transformation of LMTK− cells with purified HLA class I genes. Immunogenetics 18, 65–77 (1983). https://doi.org/10.1007/BF00401357

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  • DOI: https://doi.org/10.1007/BF00401357

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