Abstract
Flocculation of phytoplankters into large, rapidly sinking aggregates has been implicated as a mechanism of vertical transport of phytoplankton to the sea floor which could have global significance. The formation rate of phytoplankton aggregates depends on the rate at which single cells collide, which is mainly physically controlled, and on the probability of adhesion upon collision (=coagulation efficiency, stickiness), which depends on physico-chemical and biological properties of the cells. We describe here an experimental method to quantify the stickiness of phytoplankton cells and demonstrate that three species of diatoms grown in the laboratory (Phaeodactylum tricornutum, Thalassiosira pseudonana, Skeletonema costatum) are indeed significantly sticky and form aggregates upon collision. The dependency of stickiness on nutrient limitation and growth was studied in the two latter species by investigating variation in stickiness as batch cultures aged. In nutrient repleteT. pseudonana cells stickiness is very low (< 5 × 10−3), but increases by more than two orders of magnitude as cell growth ceases and the cells become nutrient limited. Stickiness ofS. costatum cells is much less variable, and even nutrient replete cells are significantly sticky. Stickiness is highest (> 10−1) forS. costatum cells in the transition between the exponential and the stationary growth phase. The implications for phytoplankton aggregate formation and subsequent sedimentation in the sea of these two different types of stickiness patterns are discussed.
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Ali, W., O'Melia, C. O., Edzwald, J. K. (1984). Colloidal stability of particles in lakes: measurement and significance. Wat. Sci. Tech. 17: 701–712
Alldredge, A. L., Gotschalk, C. (1989). Direct observations of the flocculation of diatom blooms: characteristics, setting velocities and formation of diatom aggregates. Deep-Sea Res. 36: 159–171
Alldredge, A. L., Silver, M. W. (1988). Characteristics, dynamics and significance of marine snow. Prog. Oceanogr. 20: 41–82
Biddanda, B. A. (1985). Microbial synthesis of macroparticulate matter. Mar. Ecol. Prog. Ser. 20: 241–251
Bodungen, B. V., Brockel, K. V., Smetacek, V., Zeitzschel, B. (1981). Growth and sedimentation of the phytoplankton spring bloom in the Bornholm Sea (Baltic Sea). Kieler Meeresforsch. 5: 49–60
Bodungen, B. V., Smetacek, V. S., Tilzer, M. M., Zeitzschel, B. (1986). Primary production and sedimentation during spring in the Antarctic Peninsula region. Deep-Sea Res. 33: 177–194
Calleja, G. B. (1984). Microbial aggregation. CPC Press Inc., Boca Raton, Florida
Camp, T. R., Stein, P. C. (1943). Velocity gradients and internal work in fluid motion. J. Boston Soc. Civ. Eng. 30: 219–237
Cole, J. J., Findlay, S., Pace, M. L. (1988). Bacterial production in fresh and saltwater ecosystems: a cross-system overview. Mar. Ecol. Prog. Ser. 43: 1–10
Cullen, J. J. (1982). The deep chlorophyll maximum: comparing vertical profiles of chlorophyll a . Can. J. Fish. aquat. Sciences 39: 791–803
Degens, E. T., Ittekot, V. (1984). A new look at clay-organic interactions. Mitt. Geol.-paläont. Inst. Univ. Hamburg (SCOPE/UNEP Sdbd.) 56: 229–248
Edzwald, J. K., Upchurch, J. B., O'Melia, C. O. (1974). Coagulation in estuaries. Env. Sci. Technol. 8: 58–63
Fowler, S. W., Knauer, G. A. (1986). Role of large particles in the transport of elements and organic compounds through the oceanic water column. Prog. Oceanogr. 16: 147–194
Gibbs, R. J. (1983). Effect of natural organic coatings on the coagulation of particles. Envir. Sci. Technol. 17: 237–240
Goldmann, J. C. (1987). On phytoplankton growth rates and particulate C:N:P ratios at low light. Limnol. Oceanogr. 31: 1358–1363
Guillard, R. L., Ryther, J. H. (1962). Studies of marine planktonic diatoms. 1.Cyclotella nana Hustedt, andDetonula confervacea (Cleve) Gran. Can. J. Microbiol. 8: 229–239
Hobbie, J. E., Daley, R. J., Japser, S. (1977). Use of Nucleopore filters for counting bacteria by fluorescence microscopy. Appl. envirl. Microbiol. 33: 1225–1228
Jackson, G. A. (1990). A model of the formation of marine algal flocs by physical coagulation processes. Deep-Sea Res. (in press)
Joiris, C. and co-authors (1982). A budget of carbon cycling in the Belgian coastal zone: relative roles of zooplankton, bacterioplankton and benthos in the utilization of primary production. Neth. J. Sea Res. 16: 260–280
Kiørboe, T., Kaas, H., Kruse, B., Møhlenberg, F., Tiselius, P., Ærtebjerg, G. (1990). The structure of the pelagic food web in relation to water column structure in the Skagerrak. Mar. Ecol. Prog. Ser. 59: 19–32
Kranck, K., Milligan, T. (1980). Macroflocs: production of marine snow in the laboratory. Mar. Ecol. Prog. Ser. 3: 19–24
Kranck, K., Milligan, T. G. (1988). Macroflocs from diatoms: in situ photography of particles in Bedford Basin, Nova Scotia. Mar. Ecol. Prog. Ser. 44: 183–189
LeFévre, J. (1986). Aspects of the biology of frontal systems. Adv. mar. Biol. 23: 163–299
Leussen, W. van (1988). Aggregation of particles, settling velocities of mud flocs. A review. In: Dronkers, J., Leussen, W. van (eds.) Physical processes in estuaries. Springer-Verlag, Berlin, p. 347–403
Logan, B. E., Alldredge, A. L. (1989). Potential for increased nutrient uptake by flocculating diatoms. Mar. Biol. 101: 443–450
McCave, I. N. (1984). Size spectra and aggregation of suspended particles in the deep ocean. Deep-Sea Res. 31: 329–352
McLachlang, J., McInnes, A. G., Palk, M. (1965). Studies on the Chitan (chitin: poly-n-acetylglucosamine) fibers of the diatomThalassiosira fluviatilis Hustedt. Can. J. Bot. 43: 707–713
Prieur, L., Legendre, L. (1988). Oceanographic criteria for new phytoplankton production. In: Rothschild, B. J. (ed.). Toward a theory on biological-physical interactions in the world ocean. Kluwer Academic Publishers, Dordrecht, p. 71–112
Riebesell, U. (1989). Comparison of sinking and sedimentation rate measurements in a diatom winter/spring bloom. Mar. Ecol. Prog. Ser. 54: 109–119
Rose, A. H. (1984). Physiology of cell aggregation: flocculation bySaccharomyces cerevisiae as a model system. In: Marshall, K. C. (ed.) Microbial adhesion and aggregation. Springer Verlag, Berlin, p. 323–335
Shanks, A. L., Edmonson, E. W. (1989). Laboratory-made artificial marine snow: a biological model of the real thing. Mar. Biol. 101: 463–470
Smayda, T. J., Boleyn, B. J. (1966). Experimental observations of flotation in marine diatoms. II.Skeletonema costatum andRhizosolenia setigera. Limnol. Oceanogr. 11: 18–34
Smetacek, V. S. (1985). Role of sinking in diatom life-history cycles: ecological, evolutionary and geological significance. Mar. Biol. 84: 239–251
Weilenmann, U., O'Melia, C. R., Stumm, W. (1989). Particle transport in lakes: models and experiments. Limnol. Oceanogr. 34: 1–18
Yamazaki, H., Osborn, T. R. (1988). Review of oceanic turbulence: implications for biodynamics. In: Rothschild, B. J. (ed.) Toward a theory on biological-physical interactions in the world ocean. Kluwer Academic Publishers, Dordrecht, p. 215–234
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Communicated by T. Fenchel, Helsingør
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Kiørboe, T., Andersen, K.P. & Dam, H.G. Coagulation efficiency and aggregate formation in marine phytoplankton. Mar. Biol. 107, 235–245 (1990). https://doi.org/10.1007/BF01319822
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DOI: https://doi.org/10.1007/BF01319822